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Page 1

a2) United States Patent

Guo et al.

US 10,876,193 B2
Dee. 29, 2020

US010876193B2

(10) Patent No.:
(45) Date of Patent:

(54)

(75)

(73)

@1)
(22)

(65)

(63)

(651)

(52)

(58)

NANOSTRUCTURED MATERIALS,
METHODS, AND APPLICATIONS

Inventors: Chunlei Guo, Rochester, NY (US);
Anatoliy Y. Vorobyey, St. Catharines
(CA)

Assignee: UNIVERSITY OF ROCHESTER,

Rochester, NY (US)

Notice: Subject to any disclaimer, the term of this

patent is extended or adjusted under 35
USC. 154(b) by 523 days.

Appl. No.: 13/604,951
Filed: Sep. 6, 2012

Prior Publication Data
US 2012/0328905 Al Dec. 27, 2012

Related U.S. Application Data

Continuation-in-part of application No. 12/188,258,
filed on Aug. 8, 2008, now abandoned, which is a

(Continued)

Int. Cl.

C22F 3/00 (2006.01)

B23K 26/352 (2014.01)
(Continued)

US. Cl.

CPC ... C22F 3/00 (2013.01); B23K 26/0006

(2013.01); B23K 26/0624 (2015.10);

(Continued)

Field of Classification Search

CPC esssssseesseee w+ B32B 3/26; B32B 3/30

(Continued)

(56) References Cited
U.S. PATENT DOCUMENTS

4,361,630 A
4,972,061 A

11/1982 Johnson, Sr.
11/1990 Duley et al.

(Continued)

FOREIGN PATENT DOCUMENTS,

CN 101712102 A * 5/2010
CN 110576264 A * 12/2019
JP 2001340498 A 12/2001

OTHER PUBLICATIONS

O. Zines, K. Anselme, A. Denzer, P. Habersetzer, M. Wieland, J.
Jeanfils, P. Hardouin, D. Landolt. “Time-dependent morphology and
adhesion of osteoblastic cells on titanium model surfaces featuring
seale-resolved topography.” Biomaterials 25 (2004) 2695-27 L1.*

(Continued)

Primary Examiner — Paul A Wartalowicz

Assistant Examiner — Stephani Hill

(74) Attorney, Agent, or Firm — Kilpatrick Townsend &
Stockton LLP

(37) ABSTRACT

Methods for making a material superwicking and/or super-
wetting (superhydrophyllic) involving creating one or more
indentations in the surface of the material that have a
micro-rough surface of protrusions, cavities, spheres, rods,
or other irregularly shaped features having heights and/or
widths on the order of 0.5 to 100 microns and the micro-
rough surface having a nano-rough surface of protrusions,
cavities, spheres, rods, and other irregularly shaped features
having heights and/or widths on the order of 1 to 500
nanometers. Superwicking and/or superwetting materials
having micro-rough and nano-rough surface indentations,
including metals, glass, enamel, polymers, semiconductors,
and others.

19 Claims, 50 Drawing Sheets

Page 2

US 10,876,193 B2
Page 2

Related U.S. Application Data

continuation-in-part of application No. 11/862,449,
filed on Sep. 27, 2007, now abandoned.

(60) Provisional application No. 60/847.916, filed on Sep.

29, 2006.

(51) Int. Cl.
B82Y 40/00 (2011.01)
C2ID 8/02 (2006.01)
B23K 26/0622 (2014.01)
B23K 26/00 (2014.01)
C21D 1/09 (2006.01)
BS2Y 30/00 (2011.01)
B23K 103/12 (2006.01)
B23K 103/00 (2006.01)
B23K 103/08 (2006.01)
B23K 103/16 (2006.01)
B23K 103/14 (2006.01)
B23K 103/10 (2006.01)

(52) US. Ch
CPC.

.. B23K 26/355 (2018.08), C21D 8/0294
(2013.01); B23K 2103/08 (2018.08); B23K
2103/10 (2018.08); B23K 2103/12 (2018.08);
B23K 2103/14 (2018.08); B23K 2103/16
(2018.08); B23K 2103/42 (2018.08); B23K
2103/50 (2018.08), B23K 2103/52 (2018.08);
B23K 2103/56 (2018.08); B82Y 30/00
(2013.01); B82Y 40/00 (2013.01); C21D 1/09
(2013.01); YIOT 428/12993 (2015.01), YIOT
428/24355 (2015.01)
(58) Field of Classification Search

USPC .. .. 420/38; 428/141, 687

See application file for complete search history.

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* cited by examiner

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Page 54

US 10,876,193 B2

1

NANOSTRUCTURED MATERIALS,
METHODS, AND APPLICATIONS

RELATED APPLICATION DATA

This patent application is a continuation-in-part of, and
claims priority to U.S. application Ser. No. 12/188,258 filed
on Aug. 8, 2008, which itself is a continuation-in-part of, and
claims priority to U.S. application Ser. No. 11/862,449 filed
on Sep. 27, 2007, which further claim priority to U.S.
Provisional Application Ser. No. 60/847,916 filed Sep. 29,
2006, the subject matters of which are herein incorporated
by reference in their entireties.

FEDERALLY SPONSORED RESEARCH

This invention was made with government support under
CTS0425206 awarded by the National Science Foundation.
The government has certain rights in the invention.

BACKGROUND

1. Field of the Invention

Embodiments of the invention generally pertain to the
field of materials processing and associated processed mate-
rials. More particularly, embodiments of the invention are
directed to methods for processing materials using femto-
second duration laser pulses, applications of such methods,
and materials and/or material properties resulting from such
methods. Even more particularly, embodiments of the inven-
tion are directed to methods for altering the surface structure
of metal materials using femtosecond duration laser pulses,
applications of such methods, and materials and/or material
properties resulting from such methods, including blackened
and colored metals.

2. Description of Related Art

Although materials may be shaped or otherwise altered in
a large variety of ways including milling, machining, grind-
ing, etc., in recent years, laser-based alteration of materials
has become a common method for a variety of materials
processing applications. For example, laser alteration of
materials by high energy laser pulses has been used to both
create precise hole patterns in metals or metal films as well
as for more subtle material alterations such as texturing of
metals or metal films by the intense heating/melting/vapor-
ization effects of such high energy laser beams.

Reported methods of laser alteration of materials involves
the use of ‘short-duration’ (i.e., nanosecond (ns) and pico-
second (ps)) laser pulses. See, for example, U.S. Pat. Nos.

5,635,089 and 4,972,061. U.S. Pat. No. 6,979,798 describes 5

the use of laser pulses of preferably less than 130 femto-
seconds (fs) to specifically burn metal links on integrated
circuits. Thus the use of ultrashort (ns) duration laser pulses
for laser processing of materials may achieve results that are
different than those from longer duration (i.e., ‘short’) laser
pulses. The duration of a nanosecond laser pulse is long
enough for the pulse to interact with the material as it is
ejected from the surface. Ultra-short duration, femtosecond
(fs) laser pulses, by comparison, are not long enough in
duration to interact with the material ejected from the
surface of the irradiated substance, since the pulse ends long
before the hydrodynamic expansion of the ejected material.
Another difference between different laser pulse timescales
is that the laser-supported combustion and detonation waves
that are commonly generated in a nanosecond duration laser
pulse do not occur in an ultra-short fs laser pulse, again
offering up the possibility of materials processing effects and

0

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8

3

40

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2

resulting material parameters that may be difficult or impos-
sible to obtain with longer duration laser irradiation.

In light of the above observations, advantageous benefits
may be obtained from the use of ultra-short, femtosecond
pulses in the processing of certain materials and the altered
materials or material characteristics resulting from process-
ing with one or more fs laser pulses. Certain advantageous
benefits may also be realized by the ability to controllably
modify optical properties of a metal.

SUMMARY

Embodiments of the invention are directed to methods for
processing materials using femtosecond duration (i.e., 1-999
fs) laser pulses, applications of such methods, and altered
materials and/or material properties resulting from such
methods. Particularly advantageous aspects of the invention
are directed to methods for altering the surface structure of
metal and other materials by application of one or more
femtosecond duration laser pulses, applications of such
methods, and altered materials and/or material properties
resulting from such methods. According to non-limiting
aspects, methods for uniformly coloring, non-uniformly
coloring, and blackening a metal or other material, increas-
ing a material’s absorptance (up to nearly 100%), increasing
a material’s surface area, as well as altered materials exhib-
iting these characteristics are disclosed.

As used herein, the term “colored metal” will mean a
metal having a color that is due to a femtosecond laser
pulse(s)-induced nanostructuring of the surface of the metal,
but not due to artificial or applied coloring, painting, dying,
etching, polishing, anodizing, or other non-femtosecond
laser pulse(s)-induced nanostructuring of the surface of the
metal. The term “color” will have its typical meaning as well
as the practical consideration that color is observable over
the visible light spectrum. In that regard, however, one
skilled in the art will also recognize the relationship between
color and the reflectance/absorptance of light by a material,
where ‘white’ would correspond to high reflectivity and
‘black’ to high absorptivity. As further used herein, the term
“base metal” will mean the metal prior to femtosecond laser
surface structuring as it is ready to undergo femtosecond
laser pulse(s)-induced nanostructuring of the surface of the
metal, in which case it may or may not be polished.
According to the embodiments of the invention, the absorp-
tance of the base metal will always be less than the corre-
sponding absorptance of the fs laser-treated material.

Other material alterations enabled by the disclosed fem-
tosecond pulse methods result in making non-superhy-
drophyllic or non-superwicking materials superwicking and/
or superhydrophyllic (superwetting). Wetting properties of a
solid surface are characterized by the so-called ‘contact
angle’ of a water/liquid drop deposited on the horizontal
surface of the solid as shown in FIG. 37 below. The surface
can be smooth or structured. As used herein, a surface or
material is considered ‘hydrophilic’ when it exhibits the
water contact angle smaller than 90 degrees but greater than
zero or nearly a zero contact angle. The smaller the contact
angle, the better the wetting properties. As used herein, an
altered surface or material is referred to as ‘superhydro-
philic’ (r ‘superwetting’) when the water/liquid drop spreads
to zero or nearly a zero contact angle. As used herein, a
non-superhydrophilic surface or material may be considered
to have ‘wicking’ properties when the surface or material
exhibits capillary action. Such capillary or wicking action
will, however, typically be weak or negligible. As used

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US 10,876,193 B2

3

herein, when an altered superhydrophilic surface or material
exhibits strong capillary action it is referred to as a ‘super-
wicking’ surface or material.

In accordance with the enabled superwetting and/or
superwicking phenomena disclosed herein, an embodiment
of the invention is a method for making a non-superhy-
drophyllic material superhydrophilic (superwetting). In an
exemplary, non-limiting aspect, the method includes the
steps of a) providing a non-superhydrophilic material having
at least one of a smooth, a roughened, and a pre-structured
surface and b) processing the surface region of the material
using an energy flux in the form of one or more femtosecond
duration energy pulses from a suitable energy source or
continuous radiation from a continuous energy source, and
further including creating one or more indentations in the
surface, wherein the one or more indentations have a micro-
rough surface having at least one protrusion, cavity, sphere,
rod, groove, or other irregularly shaped feature having a

height and/or width on the order of 0.5 to 100 microns, 3

further wherein the micro-rough surface has a nano-rough
surface including at least one protrusion, cavity, sphere, rod,
groove, or other irregularly shaped feature having a height
and/or width on the order of 1 to 500 nanometers. In various
further non-limiting, exemplary aspects, the embodied
method may include one or more of the following steps,
features, and/or characteristics:
wherein step (a) further comprises providing a metal
material, a glass material, a dielectric material, a semicon-
ductor material, a polymer material, a dentin material, an
enamel material, or a material comprising hydroxyapatite;
wherein step (b) further comprises creating one or more
discrete, adjacent indentations each having a maximum
surface dimension between about 0.1 ym to 5 cm;
further comprising creating a plurality of the discrete,
adjacent indentations in a selected pattern;
wherein step (b) further comprises creating one or more
immediately adjacent indentations each having a maximum.
surface dimension between about 0.1 jum to 5 cm;
further comprising creating a plurality of the immediately
adjacent indentations in a selected pattern;
wherein at least some of the plurality of the immedi-
ately adjacent indentations are overlapping:
wherein step (b) further comprises creating one or more
grooves each having a width from 10 nm to 5 mm and a
depth from 10 nm to 5 mm;
further comprising creating the one or more grooves in a
desired pattern;
further comprising creating a two-dimensional array of
grooves;
further comprising creating the one or more grooves in
straight lines;
further comprising creating a plurality of the grooves
characterized by a periodicity from 10 nm to 10 cm;
wherein the material is metal, wherein the grooves have
a periodicity from 10 nm to 10 cm, a width from 10
nm to 5 mm, and a depth from 10 nm to 5 mm;
wherein the grooves have a periodicity 100+5 jum, a
width of 100+5 um, and a depth of 75+5 ym,
further wherein the micro-rough surface com-
prises at least one of protrusions, cavities, spheres,
rods, and other irregularly shaped features on the
order of 0.5 to 10 microns, further wherein the
micro-rough surface has a nano-rough surface
comprising at least one of protrusions, cavities,
spheres, rods, and other irregularly shaped fea-
tures on the order of 5 to 500 nanometers;

10

8

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4

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4

wherein the material is glass and the grooves have a
periodicity from 10 nm to 10 cm, a width from 10 nm

to 5 mm, and a depth from 10 nm to 5 mm;
wherein the grooves have a periodicity of 100+5 um,
a width of 100+5 jum, and a depth of 40+5 ym,
further wherein the micro-rough surface com-
prises at least one of protrusions, cavities, spheres,
rods, and other irregularly shaped features on the
order of 0.5 to 10 microns, further wherein the
micro-rough surface has a nano-rough surface
comprising at least one of protrusions, cavities,
spheres, rods, and other irregularly shaped fea-

tures on the order of 5 to 500 nanometers;
wherein the material is dentin and the grooves have a
periodicity from 10 nm to 2 mm, a width from 10 nm

to 2 mm, and a depth from 10 nm to 2 mm;
wherein the grooves have a periodicity of 95+5 um,
a width of 9545 wm, and a depth of 100+5 jum,
further wherein the micro-rough surface com-
prises at least one of protrusions, cavities, spheres,
rods, and other irregularly shaped features on the
order of 0.5 to 10 microns, further wherein the
micro-rough surface has a nano-rough surface
comprising at least one of protrusions, cavities,
spheres, rods, and other irregularly shaped fea-

tures on the order of 5 to 500 nanometers;
wherein the material is enamel and the grooves have a
periodicity from 10 nm to 2 mm, a width from 10 nm

to 2 mm, and a depth from 10 nm to 2 mm;
wherein the grooves have a periodicity of 100+5 um,
a width of 100+5 jm, and a depth of 120+5 jum,
further wherein the micro-rough surface com-
prises at least one of protrusions, cavities, spheres,
rods, and other irregularly shaped features on the
order of 0.5 to 10 microns, further wherein the
micro-rough surface has a nano-rough surface
comprising at least one of protrusions, cavities,
spheres, rods, and other irregularly shaped fea-

tures on the order of 5 to 500 nanometers;
wherein the processing step is selected from at least one
of direct laser ablation, interferometric laser ablation, near-
field laser ablation, a mask projection ablation technique,
laser-assisted chemical etching, deposition from a laser
ablation plume, plasmonic nanoablation, through a self-
assembled microlens array formed by deposition of glass

microspheres on the material surface.

A related embodiment of the invention is a structured
(engineered) material that has superwicking and/or super-
wetting properties. In an exemplary, non-limiting aspect, the
material includes an initially non-superhydrophilic material
having a smooth, roughened, or pre-structured surface and
one or more indentations in the surface, wherein the one or
more indentations have a micro-rough surface including a
protrusion, cavity, sphere, rod, groove, or other irregularly
shaped feature having a height and/or width on the order of
0.5 to 100 microns, further wherein the micro-rough surface
has a nano-rough surface having a protrusion, cavity, sphere,
rod, groove, or other irregularly shaped feature having a
height and/or width on the order of 1 to 500 nanometers. In
various further non-limiting, exemplary aspects, the embod-
ied material may include one or more of the following
features and/or characteristics:

wherein the material is a metal, glass, dentin, dielectric,
semiconductor, polymer, or enamel material or a material
comprising hydroxyapatite including bones and teeth;

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US 10,876,193 B2

5

wherein the one or more indentations further include one
or more discrete, adjacent indentations each having a maxi-
mum surface dimension between about 0.1 tum to 5 cm;
wherein the one or more adjacent indentations are dis-
posed in a selected pattern;
wherein the one or more indentations further include one
or more immediately adjacent indentations each having a
maximum surface dimension between about 0.1 jum to 5 cm;
wherein the one or more immediately adjacent indenta-
tions are disposed in a selected pattern;
wherein at least some of the plurality of the immedi-
ately adjacent indentations are overlapping:
wherein the one or more indentations are grooves each
having a width from 10 nm to 5 mm and a depth from
10 nm to 5 mm;
wherein the one or more grooves are disposed in
straight lines;
wherein a plurality of the grooves are characterized
by a periodicity from 10 nm to 10 em;

wherein the material is metal, wherein the grooves have 2:

a periodicity from 10 nm to 10 cm, a width from 10
nm to 5 mm, and a depth from 10 nm to 5 mm;
wherein the grooves have a periodicity of 100+5 wm,
a width of 10045 wm, and a depth of 75+5 jum,
further wherein the micro-rough surface com-
prises at least one of protrusions, cavities, spheres,
rods, and other irregularly shaped features on the
order of 0.5 to 10 microns, further wherein the
micro-rough surface has a nano-rough surface
comprising at least one of protrusions, cavities,
spheres, rods, and other irregularly shaped fea-
tures on the order of 5 to 500 nanometers.
wherein the material is glass and the grooves have a
periodicity from 10 nm to 10 cm, a width from 10 nm
to 5 mm, and a depth from 10 nm to 5 mm;
wherein the grooves have a periodicity of 100+5 um, a
width of 100+5 «um, and a depth of 40+5 jum, further
wherein the micro-rough surface comprises at least
one of protrusions, cavities, spheres, rods, and other
irregularly shaped features on the order of 0.5 to 10
microns, further wherein the micro-rough surface
has a nano-rough surface comprising at least one of
protrusions, cavities, spheres, rods, and other irregu-
larly shaped features on the order of 5 to 500
nanometers;
wherein the material is dentin and the grooves have a
periodicity from 10 nm to 2 mm, a width from 10 nm
to 2 mm, and a depth from 10 nm to 2 mm;
wherein the grooves have a periodicity of 95+5 jum, a

width of 95+5 jum, and a depth of 100+5 jum, further 5

wherein the micro-rough surface comprises at least
one of protrusions, cavities, spheres, rods, and other
irregularly shaped features on the order of 0.5 to 10
microns, further wherein the micro-rough surface
has a nano-rough surface comprising at least one of
protrusions, cavities, spheres, rods, and other irregu-
larly shaped features on the order of 5 to 500
nanometers;
wherein the material is enamel and the grooves have a
periodicity from 10 nm to 2 mm, a width from 10 nm
to 2 mm, and a depth from 10 nm to 2 mm:
wherein the grooves have a periodicity of 100+5 jun, a
width of 100+5 um, anda depth of 120+5 uum, further
wherein the micro-rough surface comprises at least
one of protrusions, cavities, spheres, rods, and other
irregularly shaped features on the order of 0.5 to 10
microns, further wherein the micro-rough surface

a

8

35

40

6

has a nano-rough surface comprising at least one of
protrusions, cavities, spheres, rods, and other irregu-
larly shaped features on the order of 5 to 500
nanometers.

BRIEF DESCRIPTION OF THE DRAWINGS,

FIG. 1 is a graph that plots the absorptance of a gold (Au)
surface against number of fs laser pulses of varying fluence
from a Ti:sapphire laser having a central wavelength of 0.8
um and a pulse duration of 60 fs, and further shows four
regimes for absorptance change: AB, BC, CD, and DE,
according to an illustrative embodiment of the invention;

FIGS. 2A, 2B are scanning electron micrograph (SEM)
images of a gold surface (a) before irradiation and (b) after
one shot from the laser described in the legend to FIG. 1,
showing nanoscale roughness (2B) corresponding to the
region AB in FIG. 1, according to an illustrative embodi-
ment of the invention;

FIGS. 3A, 3B are SEM images of nanoscale surface
structural features produced on a gold surface (region BC of
FIG. 1) from the laser described in the legend to FIG. 1: (a)
nanobranches after two shot ablation; (b) spherical nanopar-
ticles after five shot ablation, according to an illustrative
embodiment of the invention;

FIGS. 4A, 4B are SEM images showing nanostructure-
covered, laser induced periodic surface structures (NC-
LIPSS) in an irradiated area of a sample after 20,000 shots
ata fluence of F=0.17 J/cm? (region CD in FIG. 1) from the
laser described in the legend to FIG. 1: (a) SEM micrograph
showing the period of the NC-LIPSS; (b) nanobranches and
supported spherical nanoparticles in the NC-LIPSS, accord-
ing to an illustrative embodiment of the invention;

FIGS. 5A, 5B are SEM images showing (a) NC-LIPSS on
the periphery of the irradiated area and gold-black deposit
outside the irradiated area after 10,000 shots at a fluence of
F=1.1 J/em? (region DE in FIG. 1) from the laser described
in the legend to FIG. 1; (b) gold-black deposit after 20,000
shots at F=0.17 J/cm? (region CD in FIG. 1) consisting of
spherical aggregates with a mean diameter that decreases as
the distance from the crater increases, according to an
illustrative embodiment of the invention;

FIG. 6 is an SEM image of spherical nanoparticles in a
spherical aggregate of the gold-black deposit shown in FIG.
5:

FIGS. 7A, 7B are SEM images of (A) a crater produced
by 5,000 shots at F=0.17 J/cm?; (B) SEM image of a crater
produced by 5,000 shots at F=1.1 J/cm?, according to an
illustrative embodiment of the invention;

FIG. 8 is a graph of the residual energy coefficients of
aluminum (Al) versus laser fluence following ablation with
a single 55 ns pulse from a Nd:YAG laser at various ambient
gas conditions, presented for illustrative effect;

FIG. 9 is a graph of the residual energy coefficients for
aluminum versus laser fluence following ablation with a
single 45 ns pulse of a ruby laser at various ambient gas
conditions, presented for illustrative effect;

FIGS. 10A, 10B are open-shutter photographs of plasmas
produced by 55 ns Nd:YAG laser pulses in 1 atm. air and in
vacuum at (A) F=4.7 J/cm? and (B) F=19.5 em?, where the
laser beam is normally incident on the sample from the left
(the white dashed lines indicate the front surface of the
sample), according to an illustrative embodiment of the
invention;

FIG. 11A is a graph of estimates of surface temperatures
of Al samples for a Nd:YAG laser pulse at F,,,; approxi-
mately equal to F,.=1.4 Vem? in 1 atm. air (solid line) and

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US 10,876,193 B2

7

at I’, approximately equal to F,;=2.7 J/em? in vacuum at a
base pressure of 0.01 torr (dotted line); FIG. 11B is a graph
of estimated surface temperatures of Al samples for a ruby
laser pulse at F,,, approximately equal to F,=1.1 Jiem? in 1
atm. air (solid line) and at F,,, approximately equal to
F721 Vem? in vacuum at a base pressure of 0.01 torr
(dotted line), according to an illustrative embodiment of the
invention;

FIG. 12 is a graph of the residual energy coeflicients of Al
in air at various pressures versus laser fluence following
single pulse fs laser ablation using a Ti:sapphire laser
producing 60 fs pulses with a central wavelength of about
0.8 wm at a base vacuum pressure of about 0.01 torr,
according to an illustrative embodiment of the invention;

FIG. 13A is a SEM image of a mechanically polished Al
surface before laser irradiation; FIG. 13B is a SEM image of
a typical surface modification of the Al after 1 shot at
F<F,,,,-0.053 J/cm? in 1 atm. air using the fs laser described

in the legend to FIG. 12, according to an illustrative embodi-

ment of the invention;

FIG. 14 is a SEM images of the Al surface after 1 shot at
PE; 70.086 Jcm? in 1 atm. air using the fs laser described
in the legend to FIG. 12, showing the number and size of
spherical nanoparticles on the surface being greater than
those at F=F,,; (.e., than in FIG. 13(B)), according to an
illustrative embodiment of the invention;

FIG. 15 shows open shutter photographs of plasma pro-
duced by a single fs laser pulse at F=1.16 J/cm? in vacuum
(pressure of about 0.01 torr) using the laser described in the
legend to FIG. 12, where the laser beam is normally incident
on the target from the left (the white dashed line indicates the
front surface of the sample), according to an illustrative
embodiment of the invention;

FIGS. 16(A-D) are SEM images of nanoscale structures
in the center of the irradiated spot on a copper sample
following ablation at F=0.35 J/cm? using a Ti:sapphire laser
with a central wavelength of 0.8 um and a pulse duration of
65 fs: (A) sample surface before irradiation; (B) a different
area of the copper surface after one shot ablation showing
random fine nanostructures in the form of nanoprotrusions,
nanocavities, and nanorims; (C) after two shot ablation; (D)
after 1,000 shot ablation, according to an illustrative
embodiment of the invention;

FIGS. 17(A-D) show SEM images of the central part of
the irradiated spot on copper following ablation at F=1.52
J/cm? using the laser described in the legend to FIG. 16: (A)
surface after one shot exhibiting random nanostructures in
the form of nanoprotrusions and nanocavities; (B) surface

after two shot ablation showing random nanostructures in 5

the form of spherical nanoprotrusions and nanocavities; (C)
surface after 10 shots showing both nano- and microstruc-
tures; (D) surface after 1,000 shots showing predominantly
microstructures, according to an illustrative embodiment of
the invention;

FIG. 18 shows a SEM image of copper following two shot
ablation at F=9.6 J/em? using the laser described in the
legend to FIG. 16, showing only microstructures in the
central area and nanostructures on the periphery of the
ablated spot; the insert shows microstructural details in the
central area;

FIG. 19 shows a summary graphic of the different types
of structural features observed under a SEM on a copper
surface as a function of laser fluence and number of shots,
derived using the fs duration laser pulses obtained from the
laser described in the legend to FIG. 16, according to an
illustrative embodiment of the invention;

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FIG. 20A shows an image of a copper sample surface
before irradiation; FIG. 20B shows an image of nascent
nanostructures formed on copper by ablation at F=0.35
Jem? with a single laser pulse using the laser described in
the legend to FIG. 16, according to an illustrative embodi-
ment of the invention;

FIGS. 21A, 21B are SEM images showing the evolution
of nanostructure-covered, laser induced periodic surface
structures (NC-LIPSS) in the central area of the irradiated
spot on a platinum (Pt) sample at F=0.16 J/cm? delivered
from a Ti:sapphire laser system that generates 65 fs pulses
with a central wavelength of 0.8 jum: (A) initial random
nanoroughness formed after 10 shots (the inset shows a
detailed view of the nanoroughness); (B) nanostructure-
covered LIPSS after 30 shots (the inset shows a detailed
view);

FIGS. 22(A-D) show SEM images illustrating the forma-
tion of NC-LIPSS in the peripheral area of the irradiated spot
on Pt at F=0.16 J/cm? with 100 shots using the laser
described in the legend to FIG. 21: (A) general view of
ablated spot; (B) the magnified details show that LIPSS
disappears in the central area; (C) nanostructure-covered
LIPSS with a period of 0.62 um in the peripheral area; (D)
further magnified detail of (C), according to an illustrative
embodiment of the invention;

FIG. 23 is a graph showing Atomic Force Microscopy
(AFM) measurements of the surface profile following
mechanical polishing and 10 laser shots using the laser
described in the legend to FIG. 21, according to an illustra-
tive embodiment of the invention;

FIG. 24 is a graph showing a NC-LIPSS profile measured
with AFM following 30 laser shots using the laser described
in the legend to FIG. 21, according to an illustrative embodi-
ment of the invention;

FIG. 25 is a SEM image showing nanostructure-covered
LIPSS with a period of 0.58 jum in the central area of the
irradiated spot on Au after 100 shots at a fluence of F=0.16
J/cm? using the laser described in the legend to FIG. 21,
according to an illustrative embodiment of the invention;

FIGS. 26(A-D) are SEM images of nanoroughness on a
titanium (Ti) sample following fs laser treatment at near
damage threshold fluence of F=0.067 J/cm? using a Ti:sap-
phire laser system that generates 65 fs pulses with a central
wavelength of 0.8 jum: (A) sample surface before irradiation;
(B) nanoroughness after two shot laser treatment; (C) after
10 shot treatment; (D) a magnified view of a section in (B)
showing fine surface nanostructures in the forms of nanopo-
res and nanoprotrusions typically of spherical shape, accord-
ing to an illustrative embodiment of the invention;

FIGS. 27(A-D) show SEM images illustrating the nano-
topography of Ti following femtosecond laser treatment at
F=0.084 J/cm? using the laser described in the legend to
FIG. 26: (A) nanoroughness after one shot; (B) nanorough-
ness after two shots; (C) a magnified view of a section in (A)
showing fine details of surface nanoroughness; (D) magni-
fied view of a section in (B) showing fine details of surface
nanoroughness;

FIGS. 28(A-D) show SEM images illustrating fs laser
produced periodic surface patterns on Ti following laser
treatment at F=0.067 J/cm? using the laser described in the
legend to FIG. 26: (A) periodic surface pattern after 40
shots; (B) periodic surface pattern after 100 shots; (C)
periodic surface pattern after 400 shots; (D) a magnified
view of a section in (C) showing fine details of the periodic
pattern covered with nanostructural features, according to an
illustrative embodiment of the invention;

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FIGS. 29(A-D) show SEM images illustrating fs laser
produced periodic surface patterns on Ti following laser
treatment at F=0.084 J/cm? using the laser described in the
legend to FIG. 26: (A) periodic surface pattern after 20
shots; (B) periodic surface pattern after 400 shots, (C) 5
periodic surface pattern after 800 shots; (D) a magnified
view of a section in (C) showing fine details of the periodic
pattern covered with nanostructural features, according to an
illustrative embodiment of the invention:

FIGS. 30(A-D) show SEM images illustrating the surface
nano- and microtopography of Ti following fs laser treat-
ment at F=0.16 J/cm? using the laser described in the legend
to FIG. 26: (a) nanoroughness after one shot; (b) nano- and
microroughness after 20 shots; (c) typical microroughness
covered with nanostructures after 40 shot treatment; (d)
typical columnar microstructure after 200 shot treatment,
according to an illustrative embodiment of the invention;

FIGS. 31(A-D) show SEM images illustrating the surface
topography of Ti following fs laser treatment at F=0.35
Jem* using the laser described in the legend to FIG. 26: (a) 2
nano and microroughness after one shot laser treatment; (b)
typical random microroughness covered with nanostructures
after 40 shot treatment; (c) typical columnar microstructures
after 100 shot treatment; (d) typical columnar microstruc-
tures after 200 shot treatment, according to an illustrative 2
embodiment of the invention;

FIGS. 32(A-D) show SEM images illustrating the surface
topography of Ti following fs laser treatment at F=0.48
Jem? using the laser described in the legend to FIG. 26: (a)
microroughness covered with nanoroughness after 40 shots; 3
(b) typical microstructures following 70 shot treatment; (c)
typical microstructures following 100 shot treatment; (d) a
crater with a diameter of about 350 jum after a 1,500 shot
treatment, according to an illustrative embodiment of the
invention; 3

FIGS. 33(A-D) show SEM images illustrating the surface
topography of Ti following fs laser treatment at F=2.9 J/em?
using the laser described in the legend to FIG. 26: (a) smooth
surface with microinhomogeneities after a one shot laser
treatment; (b) smooth surface with some nanostructures after
two shots; (c) a magnified view of a section in (b) showing
surface nanostructures; (d) nanotopography of a smooth
surface following 4 shot treatment with observable spherical
nanostructures as small as about 10 nm, according to an
illustrative embodiment of the invention; 4

FIG. 34 shows a plot of % reflectance versus wavelength
in nm for polished Al (open circles); “black” Al (black
diamonds; see also FIG. 35(A)); grayed Al (gray circles; see
also FIG. 35(B)); “golden” Al (gray squares; see also FIG.
35(C)); and, Al colored by NC-LIPSS (open squares; see 5
also FIG. 36), according to an illustrative embodiment of the
invention;

FIGS. 35(A-C) show photographs of metals processed to
have different optical properties: (A) black Al; (B) grayed Al
with two gray shades; (C) golden Al, according to an 55
illustrative embodiment of the invention;

FIGS. 36A, 36B show photographs of Al colored by
NC-LIPSS, where the color of the samples depend upon the
viewing angle due to a grating effect, according to an
illustrative embodiment of the invention; 6

FIG. 37 is a schematic diagram that illustrates the so-
called contact angle of a water/liquid drop deposited on the
horizontal surface of a solid;

FIG. 38: (a) Photograph of an altered glass sample; (b-d)
SEM images of microgrooves (4), and finer micro- and 6:
nanostructural features on the microgrooves [(c) and (d)],
according to exemplary aspects of the invention;

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FIG. 39 shows a 3D optical image of the surface micro-
grooves of the altered material shown in FIG, 38, according
to an illustrative aspect of the invention;

FIGS. 40(a-/) show the spreading dynamics of water on a
horizontally positioned glass sample, according to an illus-
trative aspect of the invention;

FIGS. 41(a-f) show the spreading dynamics of water on a
vertically positioned glass sample with grooves oriented
parallel to the table, according to an illustrative aspect of the
invention;

FIGS. 42(a-/) show the dynamics of water running uphill
ona vertically standing glass sample with grooves oriented
perpendicular to the table, according to an illustrative aspect
of the invention;

FIG. 43 shows a plot of uphill distance traveled by water
front vs. t"”?, according to an illustrative aspect of the
invention;

FIG, 44: (a) Photograph of a tooth with a laser-treated
area on the enamel surface; (b) 3-D optical image of the
laser-produced microgrooves; (c) and (d) SEM images
showing fine micro- and nano-roughness on the surface of
the grooves, according to an illustrative aspect of the inven-
tion;

FIG, 45: (a) Photograph of a tooth with a laser-treated
area on the enamel surface; (b) 3-D optical image of the
laser-produced microgrooves; (c) and (d) SEM images
showing fine micro- and nano-roughness on the surface of
the grooves, according to an illustrative aspect of the inven-
tion;

FIG. 46: (a) and (6) Water spreading dynamics on the
laser-treated enamel surface positioned horizontally; (c) and
(d) Water spreading dynamics on the laser-treated enamel
surface positioned vertically, according to an illustrative
aspect of the invention;

FIG. 47: (a) and (6) Water spreading dynamics on the
laser-treated dentin surface positioned horizontally; (c) and
(d) Water spreading dynamics on the laser-treated dentin
surface positioned vertically, according to an illustrative
aspect of the invention;

FIG. 48: SEM images showing typical structural features
of treated platinum surfaces; (a) and (4) Structure of parallel
grooves; (c) and (d) Micro- and nanostructural features,
according to an illustrative aspect of the invention;

FIG. 49: (a-d) Spreading dynamics of methanol on a
horizontal platinum sample, according to an illustrative
aspect of the invention; and

FIG. 50: Photographs showing methanol running uphill
ona vertically standing platinum sample; (a-d) Dynamics of
methanol running uphill; (e-/) Pictures showing transporta-
tion and accumulation of methanol to an elevated point 10
mm above the reservoir surface, according to an illustrative
aspect of the invention.

DETAILED DESCRIPTION OF NON-LIMITING,
EXEMPLARY EMBODIMENTS OF THE
INVENTION

Embodiments of the present invention are generally
directed to laser-based materials processing using one or
more femtosecond duration (i.e., 1-999.99 fs) laser pulses,
and to the altered materials obtained by such materials
processing. As used herein, the term ‘materials processing’
and ‘surface treatment’ refer to altering the surface structure
or restructuring the surface of the material being processed
by creating various nanostructures that may or may not be
created in combination with additional micro- and macro-
structures. Non-limiting examples of nanostructured sur-

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faces in accordance with embodiments and aspects of the
invention are shown in the figures and will be described in
detail below.

Material alterations obtained in accord with embodiments
of the present invention may be defined by a variety of
experimental methods for analyzing the alterations obtained
(synonymously “the materials processing outcome(s)”); for
example, by electron micrographic analysis, by spectro-
scopic analysis (e.g., absorption of light or other electro-
magnetic energy by the altered surface), and via other
techniques recognized in the art. Material alterations may
also be additionally and/or separately defined in terms of
theoretical modeling of alterations and the mechanisms by
which alterations are generated, e.g., by post-ablation rede-
position of material, by the formation of nanostructure-
covered, laser induced periodic surface structures (NC-
LIPSS), and others described herein and known in the art.

In this regard, the term “ablation” is used to refer to
material alterations generally, rather than to any specific

process of material alteration. Specifically, “ablation” is 2

defined as occurring by experimental observation, i.e., by
the onset of surface damage or alteration to the material
being processed, where the surface damage or alteration is
typically observed by eye or by SEM analysis (see, e.g.,
Example 2). Thus the term “ablation” is generic, and is not
used to refer to a specific physical process of material
alteration, for example, the specific physical process of
vaporization or other form of removal of material from a
surface, etc.

The structural material alterations described herein below
may be defined more precisely as, e.g., “nanostructures,”
“nanoscale structure,” “nanoscale roughness,” or “nanor-
oughness” obtained by femtosecond laser pulse nanostruc-
turing of the material. Other alterations that may or may not
occur in the presence of nanostructuring include, without
limitation, “microstructures,” “microscale _ structure,”
“microscale roughness,” or “microroughness” obtained by
microstructuring effects obtained by femtosecond laser
pulse nanostructuring of the material, and “macrostruc-
tures,” “macroscale structure,” “macroscale roughness,” or
“macroroughness” such as craters or other features obtained
by macrostructuring effects obtained by femtosecond laser
pulse nanostructuring of the material.

With further regard to nanostructures, terms including but
not limited to “nanobranches,” “nanoparticles,” “nanopro-
trusions,” “nanocavities,” “nanorims,” “nanopores,” nano-
spheres” are used to describe nanoscale dimension altera-
tions having the visual appearances under SEM analysis of
branches, particles, protrusions, cavities, spheres, channels,

etc. With regard to microstructures, for example, “columnar 5

microstructures” is used to refer to microstructures that
appear visually under SEM analysis as columns (see, e.g.,
FIGS. 30 and 31; FIGS. 3, 6, and 26 show illustrative
examples of these different micro- and nanostructures).

Further with regard to the above terms, SEM analysis may
be used to establish quantitative as well as qualitative
definitions for macro-, micro-, and nanostructures, and these
definitions may be used to define the materials obtained by
the materials processing methods according to embodiments
and aspects of the invention.

In some non-limiting aspects of the invention, it may be
desirable to create essentially a single kind of materials
structuring, while in other non-limiting aspects it may be
advantageous to create ‘mixed’ structuring. In this context
the word “dominated” is used herein to refer to a situation
where one type of structuring is prevalent, i.e., where one
type of structuring occurs across, e.g. about 80% or more of

5

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the surface area of the surface produced by the specified
materials processing regime. In general, however, when the
surface is “dominated” by nanostructures, for example, it
will be understood that other percentage values are explicitly
contemplated; i.e., 70, 71, 72, 73, 74, 75 . . . 97, 98, 99%
(ie., counting by 1% intervals) of the surface area is of the
structure specified.

Although the above visually-based terms are used herein
to classify nanostructuring, microstructuring, and macro-
structuring effects according to embodiments of the inven-
tion, other methods can be used to categorize these struc-
tures. For example, because the absorptance of a material is
a function of the intrinsic absorptance, Ajy7p, and the
surface roughness, A,,, alterations to a material that mani-
fest as alterations in surface roughness may be described by
absorptance changes rather than, or in addition to, descrip-
tions of macro-, micro-, or nanostructural changes based on
changes in the visual appearance. Thus Example 1 below
shows in detail how different femtosecond pulse duration
laser processing regimes alter absorptance, and how these
alterations in absorptance correlate with macro-, micro- and
nanostructural changes in the surface of the material.

Further with regard to absorptance, as discussed in
Example 1 and particularly shown in FIG. 1, the materials
processing regimes of the embodiments of the invention are
capable of producing alterations to materials resulting in
extremely high absorptivity; e.g., absorptivity for gold of
close to 100%. Such high absorptivity may have particular
utility in, eg., heat absorption applications (e.g., heat
exchange and heat absorption for hot water heating from
solar energy, etc.). However, as FIG. 1 shows, other absorp-
tance values may also be obtained. Thus the present inven-
tion is directed to producing materials having absorptance
values from 0.01, 0.02, 0.03, 0.04, 0.05, ... , 1.0 (counting
by 0.01), where the resulting absorptance of the material is
significantly greater than before processing.

The absorptance values determined in Example 1 are
measured calorimetrically; however, absorptance may also
be measured by other means, and specifically by methods
that allow absorptance to be determined as a function of the
wavelength of the light impinging on the sample. Reflec-
tivity may also be measured in addition to, or in substitution
for, absorptance, especially in situations where it is desirable
to produce a material with favorably altered reflectivity.
Reflectivity may be measured by any standard method used
for such determinations; examples of reflectivity measure-
ments are provided in, for example, U.S. Pat. No. 4,972,061,
the contents of which are incorporated herein by reference in
their entirety.

Thus in a non-limiting, exemplary aspect, materials pro-
cessing methods and resulting altered or treated materials
are directed to the field of jewelry. The surface of virtually
any metal or metal alloy such as, but not limited to, gold,
platinum, silver, stainless steel, various precious metals,
decorative metals, and others may be decorated, initialed,
patterned, colored, blackened, or otherwise marked via
femtosecond laser surface structuring so as to have, for
example, altered reflectivity ranging from the reflectivity of
the unmarked metal down to essentially 0% reflectivity,
depending upon the desired application. In various non-
limiting aspects, reflectivity may vary with wavelength
(producing different colors) and/or viewing angle.

According to the various embodiments described herein,
the materials to be altered by femtosecond laser surface
structuring include most generally all metals and alloys
thereof, including, but not limited to, gold, aluminum,
copper, platinum, titanium, tungsten, stainless steel, and

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others. The alteration of semiconductor materials and dielec-
trics are contemplated. Also contemplated are ceramic,
glass, and plastic materials.

According to non-limiting aspects, metal materials are
intended to include metal films (e.g., thin metal layers coated
on glass, silicon or other additional underlying layer) and
bulk metals. Bulk metals refer to non-thin films of more than
a few hundred nm, particularly more than | ym, and more
particularly to more than 10 jum in thickness. Thus “bulk
metals” refers to metals with the characteristics just recited,
whereas “thin films” refers to metals of less than a few
hundred nm, including the thin films described in the
Examples below.

Further with regard to the materials of the present inven-
tion, as shown in the Examples and in, e.g., FIGS. 13-14,
there is evidence that various of the alterations of the
materials obtained by the embodied materials processing
methods occur preferentially on surface defects of the mate-
rials being irradiated by the femtosecond laser pulse(s). Thus
in some aspects, highly polished materials will be used
(pre-processing) in order to reduce the preferential forma-
tion of material alterations at material defects; in other
situations, it may be advantageous to leave the material
unpolished, to roughen the material, or even to introduce
inhomogeneities or other “defects” into the material in order
to facilitate certain alterations.

Pulse duration is a function of the laser system used. In
various non-limiting embodiments, the laser system is a
Ti:sapphire laser system generating 65 fs duration pulses at
acentral wavelength of 0.8 um; however, other laser systems
generating different fs pulse durations are also contem-
plated. See, e.g., U.S. Pat. No. 6,979,798 and U.S. Publica-
tion No. 2006/0207976A1, the contents of which are incor-
porated herein by reference in their entireties, for non-
limiting descriptions of other such fs duration laser systems,
e.g., a Yb-doped fiber laser such as the FPCA uJewel™
(available from IMRA America, Ann Arbor Mich.). Other
such fs duration lasers may include, e.g., dye lasers, Cr:L-
iSAF lasers, KrF lasers, and others known in the art.

Tn addition to laser pulse duration, a number of other laser
parameters may be varied in various aspects of the present
invention in order to obtain the desired materials processing
effects, including but not limited to: the polarization of the
laser beam (typically horizontally polarized); the diameter of
the spot of laser irradiation on the surface of the material
sample (typically between 100 and 1200 ym); the wave-
length of the laser beam; the energy density, F (fluence), of
the laser beam; the number of laser pulses (shots) applied to
the material sample; the extent of overlap between multiple

laser pulses (shots) applied to the particular region of the 5

material being processed; whether the shots are applied in
vacuum or under higher pressure conditions, and others.

According to various non-limiting exemplary embodi-
ments, the fs laser has a central wavelength (lambda) of 0.8
uum. However, other wavelengths in the IR, visible, ultra-
violet, infrared, THz frequency, etc., may be advantageously
used.

With regard to laser fluence on the surface of the material
to be processed, as will be discussed below, contemplated
fluences will be sufficient to alter the surface structure of the
metal as described herein and will be generally below about
25 J/cm? at the material surface; i.c., below about 25, 24, 23,
22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6,
5, 4, 3, 2, or 1 J/cm. The exact choice of fluence varies,
however, depending upon the desired materials processing
effects. Thus for example, the summary graphic of FIG. 19
shows that different materials properties may be obtained for

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fs laser irradiation using different combinations of laser
fluence and number of laser pulses.

Further with regard to fluence, in an exemplary embodi-
ment the choice of fluence is expressed by reference to the
threshold laser fluence (synonymously, the “ablation thresh-
old” or F,,,) required for visible material surface damage
under SEM. Thus as described in the Examples, materials
processing effects can be calibrated to the ablation threshold,
e.g., the fluence specified to obtain a particular effect may be
given both in absolute terms of J/cm? or, alternatively, may
be given as a percentage of the ablation threshold, i.e., as 1,
2,3, 4,5, ..., 100, 101, 102, 103, 104, 105, . . . 10,000%
(counting by ones) of F,,;.

With regard to laser pulses, embodiments of the present
invention may use single- and multi-pulse exposures of
materials to obtain desired materials processing effects.
Laser “pulse” or synonymously, “shot”, refers to a single
laser pulse applied to the sample material using for example
an electromechanical shutter to select a single pulse. Multi-
pulse or multi-shot situations involve more than a single
shot, e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, ete. (counting by ones) up
to thousands, tens-of-thousands, or hundreds-of-thousands
of shots. The exact number of pulses or shots chosen will
depend upon the desired materials processing outcome, as
shown in for example in the summary graphic of FIG. 19 and
as discussed below.

The extent of overlap between shots in a multi-shot
situation may be varied in order to obtained desired effects,
eg., by specifying that at least x % of the area of an
additional shot or shots overlap with the first or previous
shot, where x can be 1 to 100% counting by ones (i.e., 1, 2,
3, 4,5, ... , 100%). Such variations may be particularly
important when, for example, the portion of the material in
the center of the irradiation by the laser pulse or pulses
undergoes different alterations as a result of the centrality of
the beam than portions of the material at the periphery of the
pulse or pulses (see, e.g., FIGS. 18 and 22).

As a result of shot overlap or other controllable param-
eters, a variable percentage of a surface may be altered to
have the desired structure or structures. For example, a
precise scanning pattern of a laser beam across the surface
of the material may be used to ensure that a variable
percentage of the surface is altered to possess the desired
nanostructure(s), microstructure(s), macrostructure(s), or
combinations thereof. Contemplated percentages of a sur-
face to be modified range from 1 to 100% counting by ones
(ie. 1, 2,3, 4,5,..., 100%). As shown in FIG. 18, precise
patterns of laser irradiation application, either at one fluence
alone or in a combination of fluences (e.g., high fluence/low
fluence) may influence the type of structuring of the material
obtained. FIG. 18, for example, shows that a two shot high
fluence regime at F=9.6 J/cm? on copper will produce a
mixed materials processing result of a microstructured cen-
tral area surrounded by a nanostructured periphery.

In addition to specifying the percentage of the surface to
be modified, the materials processing effects may also be
expressed in terms of a total area modified, e.g., 0.1, 0.2.,
0.3., 0.4., 0.5... .. 10,000 cm? (counting by 0.1 cm? units).
In this regard, it is advantageous that the embodied materials
processing methods produce sufficiently large amounts of
altered materials, where these amounts may be specified in
terms of the total surface area of the material that has been
altered.

The exact surface area or range of surface areas required
for any particular application of the present invention will
depend upon the application; aesthetic applications such as
jewelry, for example, will require relatively small amounts

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of altered material. In contrast, larger surface areas of altered
materials may be required for other applications, e.g., for-
mation of heat absorptive surfaces, or for applications for,
e.g., catalysis or materials implantation into the human body
for, e.g., dental implants or other situations where nano-
structuring is advantageous for cellular growth and penetra-
tion into the implant material.

According to non-limiting aspects of the invention,
advantageous nanostructured materials processing effects
may be obtained under ambient air/pressure conditions.
Thus the pressure conditions under which materials process-
ing occurs affect both the threshold laser fluence (synony-
mously, the “ablation threshold” or F,,,;) required for visible
material surface damage under SEM and the plasma ignition
threshold (f,;) as assayed by the onset of bright violet
radiation from the laser-irradiated spot as measured either by
a photomultiplier or an open-shutter camera (see, e.g., FIG.
12). Therefore, various embodiments of the present inven-
tion are directed to materials processing at: low-pressure
conditions (e.g., below 5 torr), where, for example, related
materials processing to produce “gold-black” is done;
vacuum conditions (i.e., below 0.1 torr); between 5 torr and
760 torr (1 atm), i.e., $, 6, 7, 8, 9, 10,. . . 760 torr (counting
by ones); and, at atmospheric pressure, where the Examples
provided below show desirable materials processing effects
can occur, contrary to prior teachings that materials process-
ing must be performed at low pressure.

Additionally, Example 2 below discusses the effects of
ambient air versus a highly reactive gas (oxygen) versus an
inert gas (helium) on materials processing using ns duration
pulses, and concludes that these effects are dependent upon
gas pressure, rather than the type of gas environment used.
While these effects are expected to be applicable to fs
duration pulses as well, non-limiting aspects of the present
invention nevertheless contemplate the use of purified gases
in addition to ambient air for use with the materials pro-
cessing regimes. Inert gases may have particularly desirable
effects, thus such gases or other purified gas or mixtures of
gases may be used in aspects of the present invention.

EXEMPLARY METHOD EMBODIMENTS

Embodiments of the invention use pulsed laser beams of
femtosecond (fs) duration to obtain nanostructuring of mate-
rial surfaces with or without microstructuring and/or macro-
structuring effects. The specific conditions for generating a
particular structure (macro-, micro-, or nano-) or combina-
tion of structures is a function of a number of variables
including laser pulse duration, laser energy density or flu-

ence (in J/em*), and the number of pulses or “shots” of the 5

laser beam delivered to a particular region of the material to
be altered.

As shown in the Examples below, both on theoretical and
experimental grounds, fs pulses produce different materials
processing effects than do ps and ns duration pulses. It is also
observed that the materials processing effects obtained with
fs laser pulses are not a priori uniform; rather, they depend
in part upon the specific pulse parameters such as the energy
density or fluence of the laser beam and the number of pulses
of the laser beam applied to the sample. However, various
combinations of beam fluence and shot number may advan-
tageously produce more uniform materials processing
results.

Example 1 below will describe the effects of beam fluence
and shot number on a gold sample. The results are catego-
rized into four discrete regions of effect, AB, BC, CD, and
DE as shown in FIG. 1, which shows a graph of the

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absorptance of a gold (Au) surface against number of pulses
of varying fluence from a Ti: sapphire laser (central wave-
length of 0.8 um; pulse duration of 60 fs). The different
absorptance values of the laser beam irradiated gold in
various of these regions can be correlated with differences in
the materials alterations achieved; i.e., region AB is associ-
ated with nanoscale roughness (see also FIG. 2); region BC
is associated with nanoscale roughness including nano-
branches (see FIG. 3(a)) and spherical nanoparticles (see
FIG. 3(4)) and also contains microscale structures such as
micropores, circular microgrooves, and central microchan-
nels; and, region CD contains macroscale structures such as
craters, periodic structures, and other surface deformations
(see, e.g., FIG. 7).

As Example 3 will show, the regions defined in Example
1 and shown in FIG. 1 are applicable not just to a gold
material sample but are also observed to be very consistent
across different materials. Thus Example 3 is specifically
directed to an analysis of the effects of fluence and shot
number of a fs laser beam on copper, with the SEM results
for various experiments shown in FIGS. 16-18 and 20. FIG.
19 is a summary graphic that shows that there are essentially
three regions defined by the data of Example 3: a region
dominated by nanostructures (the X region in the figure); a
region dominated by microstructures with some nanostruc-
tures (the open circle region in the figure); and, a region
dominated by macrostructures with some micro- and nano-
structures (the sold squares in the figure). These three
regions correspond to regions AB, BC, and CD (or possibly
CD/DE), respectively, of FIG. 1 and demonstrate that
although there would be no a priori ability to predict the
existence of these regions, once the regions have been
defined, the structures formed for each region are relatively
predictable.

Further confirmation of the general applicability of the
three regions of FIG. 19 to other materials including, but not
limited to, other metals, semiconductors, and dielectrics is
provided by the data of Example 5, where titanium metal
was exposed to a varying number of fs duration laser pulses
of varying fluence. Specifically, Example 5 will show that
nanostructures are present with low laser fluences (see, e.g.,
FIGS. 26-29) as expected, and that for higher fluences of,
e.g., 0.16 or 0.35 J/cm? anda sufficient number of laser shots
(e.g., 20-200; see FIGS. 30 and 31), microstructuring occurs
as predicted by the data of FIG. 19. Finally, again as
predicted by the data of FIG. 19, for 1,500 shots at a fluence
of 0.48 J/cm’, macrostructures are formed as predicted (see,

e.g., FIG. 32(d)).
Exemplary Applications

The apparatus and method embodiments described herein
may have utility in a variety of applications including, but
not limited to: aesthetic or marking applications such as the
application of patterning or coloring to the surface of
jewelry; medical applications, e.g., for implantable medical
devices, where the novel properties of the laser altered
surface of such a device may aide in, for example, integra-
tion of cells of a subject into the implant; catalysis, where the
properties of the altered materials and particularly the
increased surface area of the materials resulting from, e.g.,
nanostructuring, may improve the ability of the material to
catalyze chemical reactions; heat transfer situations, where
alterations resulting in increased absorptivity may improve,
e.g., the efficiency of solar cells and heat sinks; sensor
sensitivity, where the unique alterations to materials
described herein may be used in both a sensor’s absorbing

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element to increase the amount of electromagnetic radiation
absorbed and also in the shielding around the sensor or
sensors to protect them from various forms of stray electro-
magnetic radiation, thereby helping to improve their signal-
to-noise ratios; and, stealth technologies or other technolo-
gies where the absorption of electromagnetic energy such as
ultraviolet, visible, infrared, terahertz radiation, etc., may
cloak, conceal, or otherwise obscure the object coated or
shielded with the altered material having the desired absorp-
tive properties.
Exemplary Aesthetic or Marking Applications

A non-limiting aspect of the invention is directed to
methods for materials processing that produce altered mate-
rials for aesthetic or marking applications, for example
jewelry or other applications where the nano-, macro-, or
micro-structuring of a material’s surface may produces
desired effects.

The data of Example 1 will show that fs-based laser
processing may be used to increase the absorptivity of a

material, which can be observed visually as darkening or 2

blackening of the surface region of the material so altered.
Accordingly, an embodiment the invention is directed to a
method of obtaining the desired markings. A related embodi-
ment is directed to the materials obtained by such processing
methods.

Exemplary Biomedical Applications

A non-limiting aspect the invention is directed to a
method for materials processing that produce materials
advantageously suitable for biomedical applications, par-
ticularly medical applications where a metal or metal-clad
device is to be implanted into a subject, and alterations to the
metal or metal cladding may act to improve the biocompat-
ibility of the metal or metal cladding.

Tn this aspect, suitable materials may include metals,
ceramics, composites, and others, that are nanostructured
and/or possibly microstructured and then introduced or
implanted into a biological milieu such as in bone, in tissue,
etc., where biocompatibility is important for successful
introduction or implantation. The materials contemplated
include any as are known for introduction or implantation
into the body, and include, but are not limited to, metals such
as titanium, gold, silver, etc., alloys of these metals, com-
posites, etc. The “biological miliew” may include bone,
tissue, etc., of a whole organism, or of an isolated compo-
nent of an organism, e.g., of an isolated organ, teeth, bones,
etc. Organisms contemplated include animals, and particu-
larly mammals, including humans.

Example 5 below will discuss alterations to titanium
metal using a fs laser method described herein to alter the

surface topography of titanium for better biocompatibility, 5

ie. to provide a surface containing, e.g., pits, pillars, steps,
etc., or other structural features that serve as anchors or other
attachment, scaffolding, or stimuli for protein and/or cellular
integration.

“Biocompatibility” as used herein refers generally to
alterations in the surface of a material that increase the
ability of that material to integrate into the body, e.g.,
increase structural integration such as by invasion or inter-
penetration of the material by cells of the body or proteins
or other biological material. Biocompatibility also refers to
alterations that increase integration by decreasing rejection
of the material by the body, as would occur if the material
fails to integrate, i.e. so that the body recognizes the
material as non-integrated and thus acts to encapsulate or
otherwise reject it.

Biocompatibility may be assayed in a variety of ways. For
implants, for example, biocompatibility may be determined

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by assaying the mechanical strength or stability of the
integration of the implant into the body. Thus for example,
in osseointegration of dental implants, biocompatibility may
be assayed by determining the force required to displace or
separate out the implant from the surrounding bone. Bio-
compatibility may also be determined by directly observing
(e.g., by SEM) the extent to which proteins, cells, or other
biological materials are able to invade or integrate into the
metal or other material altered by the materials processing
methods of the present invention. As another non-limiting
example of an assay for biocompatibility, methods for
measuring cell death or proliferation may be used to deter-
mine the extent to which the altered surface topography of
the material processed by the laser methods of the present
invention results in the activation of cells to proliferate, or
the active suppression of cell death mechanisms that would
otherwise occur if the cells failed to find themselves in a
suitable proliferative environment.

Exemplary Catalysis Applications

A non-limiting aspect is directed to a method for fs laser
pulse materials processing that can produce materials with
desirable catalytic properties; i.e., materials that contain
nanostructural and macro- and/or micro-structured altera-
tions that increase catalytic surface area.

Such alterations may be assayed by SEM or other analy-
ses that allow for the determination of the porosity or other
increased surface area aspects of the materials altered.
Alternatively, catalytic activity may be measured directly by
determining the rate at which a reaction is catalyzed by an
unaltered material (e.g., platinum) versus the rate of the
reaction using an altered material.

Exemplary Modifications of the Optical Properties of Mate-
rials

An aspect of the invention is directed to a method for
altering the optical properties of materials, including, but not
limited to, metals such as are provided in Example 6 below.
Thus as shown in Example 6, the materials processing
methods of the present invention may be used to obtain, e.g.,
metals which appear to the human observer to have various
shades of gray (where “gray” may alternatively be defined as
a material having relatively uniform reflectance across the
entire visible wavelength), including multiple shades of gray
in one metal piece. These materials processing methods may
additionally be used to obtain what appear to the human
observer to be colored materials (where “colored” may
alternatively be defined as a material having preferential
reflectance in some regions of the visible spectrum and not
in others), e.g., colored metals such as are also described in
Example 6, and ‘black’ metal. Although these methods are
applied to metals in Example 6, the present invention
explicitly contemplates the application of these methods for
certain non-metal materials as well.

Example |

Experiments in support of embodiments of the invention
have demonstrated that a significant amount of residual
thermal energy is deposited in metal samples following
multi-shot femtosecond laser ablation. Traditionally, it was
commonly believed that one of the most important advan-
tages of femtosecond laser ablation is that the energy depos-
ited by ultrashort laser pulses does not have enough time to
move into the bulk sample; therefore, the residual thermal
energy remaining in the bulk sample should be negligible. In
contrast to this, a significant enhancement in laser light
absorption was observed recently by the inventors following
ablation. To understand the physical mechanisms of laser

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energy absorption, the change in absorptance of gold due to
structural modifications following multi-shot femtosecond
laser ablation was directly measured. The measured data
indicates that there is a significant absorption enhancement
due to nanostructuring in addition to the known mechanisms 5
of absorption increase via micro- and macro-structuring.
Moreover, nanostructuring alone may enhance the absorp-
tance by a factor of about three. The physical mechanism of
the total enhanced absorption is due to a combined effect of
nano-, micro-, and macro-structural surface modifications
induced by femtosecond laser ablation. At a sufficiently high
fluence and with a large number of applied pulses, the
absorptance of gold surface may reach an absorptance value
of nearly 100%.

The absorptance A of a pure metal with a clean surface
consists of two components A=AyyrptAge, Where Apyrp is
the intrinsic absorptance and Ag, is the contribution due to
surface roughness. For an optically smooth metal surface,
Aga is about 1-2% of Ayre, but the role of Asz enhances as
the surface roughness increases. For multi-pulse ablation,
only the first femtosecond laser pulse interacts with an
undamaged surface, since the laser-induced surface struc-
tural modification develops long after the ultrashort pulse. In
this case, A is governed by Ajx7g, Which can be a function
of laser fluence due to laser-induced change in the dielectric
constant of the material. All of the subsequent laser pulses
interact with a structurally modified surface and their
absorption is determined by both Ajjrp and Asp. The
absorption of a single femtosecond laser pulse by an undam-
aged metal surface is dominated by Ajy7p. However, the 3
coupling of laser energy to a metal in multi-pulse femto-
second laser ablation has not yet been investigated, where
Asp may have a significant value due to surface structural
modification.

The instant non-limiting example discusses the effect of 35
surface structural modifications on the absorptance of gold
in multi-pulse femtosecond laser ablation when an originally
plane and smooth surface transforms into a blind hole. This
effect is investigated as a function of the number of applied
ablation pulses at various fluences. A reported calorimetry
technique allows a direct measurement of laser energy
absorbed by the sample. Our data indicate that femtosecond
laser-induced surface modification enhances the sample
absorptance, which can reach a value close to 100% at a
sufficiently high fluence with a large enough number of 4:
applied pulses. Scanning electron microscope (SEM) studies
show that there is absorption enhancement due to nanostruc-
turing, which, alone was seen to enhance the absorptance by
a factor of about three.

Experimentally, an amplified Ti: sapphire laser system 5
was used to generate 60-fs pulses of about 1.5 mJ/pulse at
1 kHz repetition rate at a central wavelength of 800 nm. The
laser beam was focused onto a mechanically polished
sample surface with a 40-cm-focal-length lens at normal
incidence. An electromechanical shutter was used to select
the number of pulses, N, applied to the sample. The absorp-
tance of the ablated spot was studied. After ablation of the
sample with a chosen number of pulses, we reduced the laser
fluence to a level below the ablation threshold. Subse-
quently, we irradiated the ablated spot again using a train of 60
low-fluence laser pulses that would not induce any further
surface modification. A certain amount of energy from this
low-fluence pulse train, E,, is absorbed in the skin layer of
the sample, dissipates via heat conduction in the sample, and
causes a bulk temperature rise AT. We measured this tem- 6
perature rise with a thermocouple battery that allows E, to
be determined calorimetrically as E,=CAT, where C is the

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20

known heat capacity of the sample. To measure energy E,
incident upon the sample, a certain fraction of incident pulse
train energy was split off by a beam splitter and measured
with a joulemeter. Having measured E, and E.,, the absorp-
tance of the ablated spot could be found as A=E,,/E;,.
Laser-induced surface modifications were then studied using
a SEM and an optical microscope.

The optical properties of surface modifications were stud-
ied following multi-pulse ablation at single-pulse laser flu-
ences of F=1.1, 0.35, 0.17, and 0.078 J/cm? in air. The
ablation threshold F,,, for a pristine surface was found to be
F.,,,-0.067 and 0.048 J/cm? for single-pulse and 500-pulse
train irradiation, respectively. The numbers of pulses
required to perforate a 1 mm-thick sample at the center of
the irradiated spot were determined to be 16,100, 25,000,
and 77,000 pulses at F=1.1, 0.35, and 0.17 J/cm*, respec-
tively. This corresponds to average ablation rates of 63, 40,
and 13 nm/pulse, indicating that a single laser pulse pro-
duces a nanoscale modification in depth. Plots of absorp-
tance versus the number of ablation shots, N, at different F
are shown in FIG. 1. For an undamaged surface, the absorp-
tance remains a constant value of 0.12 when measured at
F=0.0043 cm?, which is an order of magnitude below F.,,,,.
The absorptance of a structurally modified surface is sig-
nificantly greater than that of the undamaged surface and
shows dependence on the number of applied ablation pulses,
N.

The A(N) curves for the ablated surface can be charac-
terized into distinct regions marked with A, B, C, D, and E
on A(N) in the case of F=0.17 J/cm? in FIG. 1. The first of
these four regions is region AB, where the absorptance
initially increases from 0.12 (undamaged surface) to a value
in the range of 0.25-0.33. Typically, this region covers the
first 1-10 shots. For example, this initial enhancement of
absorptance can be produced by four pulses at F=0.17 Jem?
or by one pulse at F=0.35 and 1.1 J/em?. Optical microscopy
showed that the irradiated spot was entirely covered with
surface modification following ablation by only one pulse
when F20.35 J/cm’, but four pulses at F=0.17 J/cm. There-
fore, the enhancement of A with N at F=0.17 J/cm? appears
due to both the surface modification and an increase in size
of the modified area from point A to B.

In the second region, BC, the absorptance undergoes a
slight decrease as N increases. Typically, this region covers
approximately the next 100-300 pulses. Both regions AB
and BC extend to a larger number of pulses when the surface
is modified at F only slightly above F,,,, as seen from the
curve at F=0.078 em? in FIG. 1.

The third region, CD, is characterized by a further
enhancement of absorptance with the increase of N. This
region extends to N on the order of 10,000 pulses.

The fourth region, DE, was where absorptance reached a
maximum value that did not change with further increase of
N.

Reference is now made to the SEM pictures of surface
morphology shown in FIGS. 2-6. In regions AB, BC, and
CD, where absorptance exhibits dependence on N, the
following surface modifications were observed: For region
AB, a characteristic modification is nanoscale roughness
(FIG. 2). In region BC, two major features were observed.
First, nanoscale roughness develops further in the form of
nanobranches (FIG. 3 (a)) and spherical nanoparticles (FIG.
3 (b)). Secondly, microscale structures begin to develop in
the forms of micropores, circular microgrooves, and central
micro-channels. In region CD, features resembling a crater
with a deep central micro-channel, periodic structures with
orientation in the direction perpendicular to the laser light

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polarization and with a period roughly equal to the laser
wavelength (FIG. 4(a)), and a visible black halo around the
crater were observed. All these laser-induced surface modi-
fications can affect the absorptance in various ways. For
example, surface roughness can enhance the absorption of
light both by multiple reflections in micro-cavities and by
variation in the angle of incidence (angular dependence of
Fresnel absorption). Nanoscale structural features can affect
absorptance since the optical properties of a nanostructured
material can be quite different from the bulk. Laser-induced
periodic surface structures (LIPSS) may enhance absorption
of laser energy via generation of surface electromagnetic
waves. In accordance with non-limiting aspects of the
instant invention, the observed LIPSS, referred to herein as
nano-structure-covered laser-induced periodic surface struc-
tures (NC-LIPSS) having non-conventional, finer nanoscale
structural features, are shown in FIG. 4(6).

The absorption of laser energy in femtosecond laser
ablation may also be altered through re-deposition of ablated

material. Examination of the black halo produced around the 2

crater shows that its elemental composition as determined by
energy dispersive X-ray analysis is identical to that for a
pristine surface; i.e., the black halo is a layer of the ablated
and re-deposited gold. SEM images in FIGS. 5 and 6
demonstrate that the black halo has a structure of spherical
nanoparticle aggregates that is typically seen in gold-black
films and, which, have been known for their high absorp-
tance in the infrared. Therefore, the gold-black halo can
enhance the absorption of low-intensity wings of the inci-
dent Gaussian beam and contribute to residual heating of the
sample. Since re-deposition of ablated material occurs both
outside and within the ablated spot, the re-deposition of the
nanoparticles produced by ablation can also enhance the
absorption of light in the ablated area. For example, an
enhanced absorption of light by a semiconductor coated with
Au nanoparticles has recently been reported. Therefore, in
femtosecond laser ablation, the enhanced absorption can
occur due to surface nano-, micro-, macro-structures and
re-deposition of nanoparticles depending on ablation con-
ditions. The combined effect of these surface modifications
can lead to virtually 100% absorption of laser light in
multi-pulse ablation with a sufficiently large number of
pulses at high fluence as shown in FIG. 1. Almost all of
incident laser energy is retained in the sample as residual
thermal energy. This suggests that the energy carried away
by the ablated material is small in Au, and the enhanced
absorptance appears to be the dominant factor in the
enhanced residual thermal energy deposition in multi-pulse
femtosecond laser ablation at large numbers of applied
pulses.

Since different surface modifications are superimposed on
each other, it is difficult to completely isolate and determine
each individual contribution to the enhanced absorptance.
Therefore, we provide the following estimations on the
contributions of nano-, micro-, and macro-structures
induced by femtosecond laser ablation. Since surface nano-
structures are the dominant feature in region AB and part of
region BC for N<50-100, and the absorptance increases
from 0.12 to 0.25-0.33 over these regions (see FIG. 1),
nano-structures alone are believed to account for the addi-
tional absorptance increase of about 0.1-0.2. The contribu-
tion of two microscale structures, LIPSS and random rough-
ness, is estimated as follows. To estimate the contribution of
LIPSS, we ablated a sample using p-polarized light and
measured the low-fluence absorptance A(N) of the ablated
spot with both p- and s-polarizations. The curves A(N) of
different polarizations were identical, indicating that the

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22

grating effects of microscale LIPSS on the absorption of
laser light by gold is negligible. To estimate the contribution
of microscale random roughness, we abraded a mechani-
cally polished sample surface with sandpaper to produce a
rms roughness of 3 jum, which is estimated to be comparable
to the laser-induced roughness for 100<N<1000. The
absorptance of this abraded surface was then measured to be
about 0.24 as opposed to 0.12 for a mechanically polished
surface. This indirectly shows that the random micro-rough-
ness accounts for the additional absorptance increase of
about 0.12. Macro-structures come into play in two major
forms, deep central channel and concentric ring grooves,
when the number of pulses is between about 500-1000 and
laser fluence is higher than 0.17 J/cm?. Two SEM pictures
showing typical macro-structure craters are presented in
FIG. 7. The macro-scale crater formation starts in region CD
and, therefore, we believe the progressive increase of macro-
structure size largely accounts for the absorptance increase
from 0.4 to about 1.0. However, nano- and micro-structures
also develop further in regions CD and DE and may also
contribute to absorptance increase to some extent.

Besides the physical mechanisms of enhanced absorption,
we also make the following observations about femtosecond
laser-matter interactions: First, laser-induced nanostructures
alone can enhance the absorptance of Au by a factor of about
three following only 1-3 pulses. This result suggests a new
direction for future study of optical properties of nanostruc-
tures imprinted on a metal surface. Secondly, we produced
a new type of microscale periodic structure with much finer
nanoscale structures (NC-LIPSS) following ablation with a
large number of applied pulses. Thirdly, re-deposition of
laser-induced nanoparticles is seen outside of the ablated
spot leading to the formation of a nanostructured material
known as gold black. Finally, we identified potential new
applications of femtosecond laser ablation for modifying
optical properties of metals and producing technologically
valuable surface coatings such as, but not limited to, gold-
black films.

Example 2

In this Example, a comparative study of residual thermal
effects in aluminum following fs laser ablation was per-
formed. At laser fluences above the ablation threshold where
plasmas are produced and at a sufficiently high ambient gas
pressure, an enhanced coupling of pulsed laser energy into
the sample occurs. Furthermore, in contrast to the conven-
tional understanding that residual thermal energy is negli-
gible in fs-laser ablation, up to 70% of the incident pulse
energy can be retained in the sample following single-pulse
fs-laser ablation in 1-atm air. The major factors influencing
thermal energy coupling to the sample are the laser fluence
and ambient gas pressure. Residual thermal energy deposi-
tion decreases with reducing ambient gas pressure.

Laser ablation using femtosecond (fs) laser pulses has
numerous applications in the field of materials processing
and machining and, nanotechnology. Comparative studies
have demonstrated that femtosecond laser ablation has
advantages over nanosecond ablation in aspects of higher
precision, reduced heat-affected zone, and smaller amount of
debris around the ablated spot. Following laser ablation, a
fraction of absorbed laser energy is retained in the heat-
affected zone, dissipates into the bulk of the sample and
remains inside as residual thermal energy that induces the
bulk temperature of the sample to rise. This is sometimes
referred to as the thermal load and is often undesirable in
laser micro- and nano-machining

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The coupling of thermal energy into metals has been
previously studied for microsecond and nanosecond laser
ablation. An enhanced residual thermal energy coupling to
metals has been observed when laser fluence is above a
certain threshold value. It has been suggested that, in addi-
tion to the direct absorption of laser light, energy transfer
from laser-produced plasma can contribute to residual heat-
ing. However, mechanisms responsible for thermal coupling
are still not fully understood.

We have observed an enhanced residual heating of metals
following multi-pulse femtosecond laser ablation, where
laser-induced surface modification has been found to play a
role in enhanced residual heating but, where this alone could
not fully account for the observed amount of deposited
thermal energy. To exclude the effect of surface modification
on residual thermal response, we investigated single-pulse fs
ablation. We also studied the residual thermal response of
aluminum (Al) following ns-laser ablation to compare with
the results for fs-laser ablation. We used a calorimetric
technique to study effects of laser pulse duration, ambient
gas pressure, and laser wavelength on residual heating of Al.
To characterize the residual thermal response, we defined a
so-called residual energy coefficient (REC) K=E,/E,, where
E, is the residual thermal energy remaining in the sample
following ablation and E, is the incident laser pulse energy.
By definition, REC is equal to absorptance of the sample
material when laser fluence is below the ablation threshold.
Enhanced residual heating occurred following both single-
pulse ns- and single-pulse fs-laser ablation in ambient gas at
a sufficiently high pressure. The major factors governing the
residual heating are laser fluence and ambient gas pressure.
There is a fundamental difference between multi-pulse ver-
sus single-pulse ablation because multi-pulse ablation may
induce absorptance change due to accumulated surface
modifications from multiple laser shots. This accumulated
effect does not occur in single-pulse ablation.

Both ns and fs duration pulse effects were examined. The
following three laser systems were used: 1) a ruby laser
producing 45-ns pulses (FWHM) at wavelength 40.69 pm.
with pulse energy of 0.6 J; 2) a Nd:YAG laser generating
55-ns pulses at 4=1.06 jum with pulse energy of 1.4 J; and,
3) a Ti:sapphire laser producing 60-fs pulses at A-0.8 jum.
with pulse energy of 1.5 mJ. Using each laser system, the
laser beam was focused onto an Al sample at normal
incidence. A fraction of the incident pulse energy E, was split
off using a beamsplitter and measured with a joulemeter to
allow E, to be determined.

The residual energy Ex that remains in the sample fol-
lowing ablation causes the bulk temperature of the sample to

rise by AT. Using a thermocouple attached to the Al sample, 5

AT was measured after thermal equilibrium was reached in
the sample. Knowing the specific heat capacity c, and the
mass m of the sample, the residual energy can be obtained
from E,=mc,AT. The thermocouple response time (the time
required for achieving a maximum thermocouple signal in
our calorimeter) was about 2.5 sec. Using measured E, and
Eg, the residual thermal energy coeflicient K=E,/E, can be
found as a function of single-pulse laser fluence F=E,/S,
where S is the laser beam area on the sample. The samples
were mechanically polished. Measurements were performed
in various ambient gases and at different pressures. The
sample was translated with an X-Y stage so each subsequent
laser pulse was incident on a fresh spot. Two parameters, the
ablation threshold F’,,; and the plasma ignition threshold F,,,
were determined at the onset of surface damage visible to
eye with subsequent examination under a scanning electron
microscope (SEM). F,; was determined by observing the

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24

onset of bright violet radiation from the irradiated spot using
either a photomultiplier (PMT) or an open-shutter camera,
both properly filtered to cut off scattered laser light.

Although embodiments of the invention are directed to
fs-duration laser pulses, this Example probes some of the
effects of both fs- and ns-duration laser pulses. The depen-
dence of REC on laser fluence F following single-pulse
ns-laser ablation in various ambient gases under different
pressures on A] are plotted in FIGS. 8 (or Nd:YAG laser) and
9 (or ruby laser). For the Nd:YAG laser, ablation and plasma
ignition thresholds in l-atm air are determined to be
F,71.2#0.3 Vem? and F,,-1.4#0.4 em’. For the ruby
laser, these values are F,,,=1.00.2 J/cm? and F,p1.120.3
cm’. Thus F,,F,, in these experiments. By definition,
REC should be equal to the absorptance of the material when
it is irradiated by low-fluence laser light that does not cause
any surface modification. The measured value of REC
(K=0.25) at F<F,,, in FIG. 2 agrees with the reported value
of absorptance for a mechanically polished Al sample at
4=1.06 um (D. E. Gray (Ed.): American Institute of Physics
Handbook, 3rd edn. (McGraw-Hill, New York, 1972)), and
this agreement shows the accuracy of our measurement
technique. Data at 1-atm in FIGS. 8 and 9 show that REC
enhances abruptly at a certain fluence threshold, F,,,,, and
reaches a maximum value of about 0.5-0.6 indicating that
about 50-60% of the laser pulse energy can be retained in Al
following nanosecond laser ablation. Our experiment also
shows that F,,,,,F',, within the experimental uncertainty for
both Nd:YAG and ruby laser ablation.

Next, we studied the pressure effect on REC; representa-
tive curves are plotted in FIGS. 8 and 9, For Nd:YAG laser
ablation, REC slightly decreases when air pressure, P,
decreases from 1 atm to about 30 torr, but REC abruptly
drops when pressure further reduces from 30 torr to about
0.6 torr. For P<0.6 torr, the onset of plasma is accompanied
with a drop of REC. This drop becomes more pronounced as
the pressure is further reduced to 0.04 torr. At this pressure,
REC eventually reaches a value of about 0.12 that is smaller
than the absorptance of an undamaged surface by a factor of
two. For P<0.04 torr, REC virtually remains independent of
the residual air pressure. This behavior shows that, in
contrast to the observation in air, the onset of plasma in
vacuum is accompanied by a drop of REC. In vacuum, both
F,5; and F’,; are higher than those at 1-atm air pressure by
approximately a factor of two. Dependence of REC on laser
fluence is also studied in 1-atm oxygen and 1-atm helium,
and these REC data are shown in FIGS. 8 and 9. The
dependences show virtually the same behavior as those in
air, indicating that REC does not essentially depend on the
particular type of gas. The contribution of possible exother-
mic chemical reactions that may occur due to presence of
chemically active gases such as oxygen is negligible.

In vacuum, the laser plasma mainly consists of ionized
species of ejected material, while in a gas medium, plasma
consists of ionized species of both ablated material and
ambient gas. A characteristic feature of ambient gas plasma
produced by ns pulses is that the plasma expands due to the
generation of laser-supported absorption waves. FIG. 10
shows open-shutter photographs of plasmas produced by
55-ns Nd:YAG laser pulses for ablation of Al in both air and
vacuum under the same experimental conditions. Distinction
between plasmas can be clearly seen. The size of plasma in
air is larger than that in vacuum. Therefore, the role of
plasmas in residual heating of the sample in air may differ
from that in vacuum.

The direct absorption of laser energy is a factor that may
influence residual heating. According to the Drude model,

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when the temperature increases material absorptivity should
also increase due to an enhanced collision frequency
between free electrons and thermally vibrating lattice atoms.

Therefore, one should expect an increase in REC with laser
fluence due to this enhancement of material absorptivity.
However, the fact that REC increases in air while it
decreases in vacuum above a certain laser fluence indicates
that the temperature-enhanced Drude absorption does not
play an essential role in enhanced residual thermal response.
This is also confirmed by our estimation of the laser-induced
surface temperature using the following formula:

_ Ava prie-9)
—KVa Jo Ve

Ts(t) dO+T

where A is the absorptance, a is the thermal diffusivity, 1 is
the intensity of incident laser light, k is the thermal conduc-
tivity, t is the time, T, is the initial temperature, and @ is the
integration variable. FIG. 11(a) shows the computed T.(t)
induced by the Nd:YAG laser pulse at F,,-F,=1.4 Jem? in
1-atm air and at P,,=F,-2.7 J/em? in vacuum with A=0.25,
a=1.0x10~* m/s, k=240 Js“! m-!° C.“!, and T,=20° C. One
can see that the maximum surface temperature is about 500°
C. in air and 1000° C. in vacuum. The estimated surface
temperature in air is below both the melting (660° C.) and
boiling (2495° C.) points of Al. The computed T(t) for ruby
laser at F,,@F,-1.1 Vem? in 1-atm air and at F,,aF,-2.1
J/cm? in vacuum with A=0.28 are shown in FIG. 11(b)

Similar to the results of Nd:YAG laser in FIG. 5(a), the
estimated surface temperature for ruby laser irradiation in air
is also below both the melting and boiling points of Al. Thus
when the enhanced thermal coupling occurs in 1-atm air, the
estimated surface temperature induced by both Nd:YAG and
ruby lasers is too low to induce a significant increase in
absorptance.

The similar general behavior of REC for Nd:YAG
(A=1.06 jum) and ruby (A=0.69 jum) lasers shows that laser
wavelength is relatively unimportant in the visible and near
infrared spectral region. Nevertheless, our experiment
clearly demonstrates that REC of the aluminum sample
depends mainly on laser fluence and ambient gas pressure
following ns-laser ablation.

The dependence of REC on laser fluence for Al following
fs-laser ablation in 1-atm air and in vacuum (P=0.01 torr) are
plotted in FIG. 12. The residual thermal energy coupling is
enhanced in air above a certain threshold value of laser
fluence, while in vacuum it is reduced. The values of F
F,,, and F,,,
and 0.5 J/cm’, respectively. These thresholds are well sepa-
rated and the enhancement threshold is above the plasma
threshold; i.e. F,>F,>Fa, in contrast to the ns-laser
ablation where F,,,-F,-F,,, We note that our measured
value of F.,, in 1-atm air agrees with reported values for Al
thin film deposited on a silicon substrate. The values of F ,,;
and F,, in vacuum are determined to be 0.058 Vem? and
0.096 J/cm, respectively (see FIG. 12). Contrary to con-
ventional understanding that the residual thermal energy is
negligible in an ablated sample following femtosecond laser
ablation, our data show that REC reaches a value of 0.7
indicating that, at the highest laser fluence achievable in our
experiment (F=4 J/cm*), about 70% of the incident laser
energy can be retained in the sample following single-pulse
fs-laser ablation in 1-atm air

FIG. 13(a) shows an SEM image of an undamaged
surface that is mechanically polished. A view of the sample

aby?

, in air are found to be 0.053 J/cm, 0.086 Wem?, 5

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surface after irradiation in air at F=F,,,; is shown in FIG.
13(4). (FIG. 13(4) does not show the same spot on the
sample as in FIG. 13(a)). FIG. 13(6) shows that surface
defects are preferential spots for initial ablation with some
sparsely distributed small spherical nanoparticles in the
irradiated area. FIG. 14 shows a typical laser-induced sur-
face morphology following ablation at F=F,,, in 1-atm air. It
is seen that surface modifications are still focalized around
surface defects, but both the number and the size of nan-
oparticles are greater than those at F=F,,,. Therefore, mate-
rial ejection in fs-laser ablation appears to be initiated at
surface defects. Open-shutter photographs of the femtosec-
ond laser-induced plumes taken at F=1.16 J/cm? (higher than
F,,) are shown in FIG. 15. The figure shows that the size of
the plume in air is larger than that in vacuum (P=0.01 torr).
There are three basic distinctions between ns- and fs-laser
ablation. First, {8-laser pulses do not interact with ejected
material because hydrodynamic expansion of ablated mate-
rial from the irradiated area occurs on a timescale much
longer than femtosecond pulse duration. Secondly, laser-
supported absorption waves that are commonly generated in
ns-laser ablation in a gas medium do not exist in fs-laser
ablation. Thirdly, a material irradiated with an intense fs-
laser pulse can be heated to a solid-density plasma state.

Example 3

Unique properties of nanomaterials have been extensively
studied in the past and various nanostructures have found
numerous applications in optics including enhanced x-ray
emission and enhanced absorption in intense light—matter
interaction, and optical biosensing, to name a few. Direct
surface nanostructuring (i.e., not from ablated plume depo-
sition) may be used in a number of technological applica-
tions, for example, manipulation of optical properties of
solids, catalysts, dental implants, etc. We performed a
detailed study of the morphology of surface nanomodifica-
tions produced on bulk metals using a femtosecond laser
ablation technique embodied herein. The effects of laser
fluence and number of applied pulses on the generated
surface nanostructures were studied with a scanning electron
microscope (SEM). According to an aspect, a set of optimal
laser irradiation conditions for metal surface nanostructuring
is disclosed.

In our experiment, we used an amplified Ti:sapphire laser
system that consisted of a mode-locked oscillator and a
two-stage amplifier including a regenerative amplifier and a
two-pass power amplifier. The laser system produces 65-fs
pulses with energy around 1 mJ/pulse at a 1 kHz repetition
rate with a central wavelength A-800 nm. To produce
ablation, the laser beam is focused normally onto a bulk
sample mounted vertically. To measure the incident pulse
energy, a certain fraction of the incident light is split off by
a beam splitter and measured with a pyroelectric joulemeter.
The number of laser shots, N, applied to the sample is
controlled using an electromechanical shutter. All experi-
ments were performed in air under atmospheric pressure.
The morphology of femtosecond laser-induced surface
modifications was studied using a SEM. The studied
samples were mechanically polished copper, gold, and plati-
num. The range of laser fluence used in the ablation was
between 0.084 and 9.6 J/cm?. The number of applied pulses
was varied from 1 to 5x10* shots. The ablation threshold
was determined as the minimum fluence to generate a
surface damage seen under the SEM.

ASEM picture of a copper sample surface prior to laser
irradiation is shown in FIG. 16(a). For reference, the abla-

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27
tion threshold for a copper sample was determined to be
F,,;-0.084 J/cm? following a total of N=100 shots. The
morphology of the irradiated surface was studied following
ablation with laser fluence of F=0.084, 0.16, 0.35, 1.52, 3.7,
and 9.6 J/cm? and the number of applied pulses in the range
of 1-5x10*. A number of representative surface structures
produced on the copper sample are shown in FIGS. 16-18.
An analysis of the SEM data shows that the morphology of
femtosecond laser-induced surface nanostructures depends
both on laser fluence and the number of applied pulses. The
effect of the total number of shots on nanostructuring at
F=0.35 and 1.52 Mcm? is shown in FIGS. 16 and 17,
respectively. FIG. 16(6) shows that nanostructures begin to
occur on some random localized sites after one shot at
F=0.35 J/cm. A few larger-size structural features are also
observed in the central part of the ablated area, as seen in
FIG. 16(6). These larger structures may be associated with
surface defects and/or laser beam intensity inhomogeneities.
FIG. 16(c) shows a nanoscale surface structure produced by

two-shot ablation. The structure comprises both larger nano- 2

cavities and nanoprotrusions with spherical tips of diameter
up to about 75 nm. Therefore, the one additional shot
transforms the sparsely distributed nanoscale features in
FIG. 16(4) to the cellular-like structures in FIG. 16(c). The
surface morphology after ablation with 1000 pulses is shown.
in FIG. 16(d). One can see that the mean size of nanopro-
trusions becomes larger while at the same time some nano-
cavities develop into microcavities. The evolution of the
surface structures following ablation at F=1.52 J/cm? and
various N is shown in FIG. 17(a-d). At this middle fluence,
pure nanostructures are only generated by ablation with one
or two laser shots (FIGS. 17(a) and 17()). As shown in FIG.
17(c), 10 shot ablation produces both random nano- and
micro-structures. With further increasing N, the proportion
of nanostructures decreases as can be seen in FIG. 17(d),
where microscale structures become dominant. At the high-
est fluence used in our experiment, nanostructures are not
present over most of the irradiated area and a dominant
morphological feature is microroughness. However, nano-
structuring can still be observed on the periphery of the
ablated spot where the Gaussian beam intensity is low
enough for nanostructural formation. An example of these
surface structural modifications is shown in FIG. 18 for
two-shot ablation at F=9.6 J/cm?.

The effect of laser fluence on surface structuring can be
seen from analyzing the surface modifications produced at
various F and fixed N as shown for example in FIG. 16(c)
(F=0.35 I/em?, N=2), FIG. 17(6) (F=1.52 J/em*, N=2) and
FIG. 18 (F=9.6 Iem?, N=2). These images show that

ablation with high laser fluence does not actually induce 5

nanostructures and therefore there exist optimal laser abla-
tion conditions for surface nanostructuring. In order to
determine the optimal conditions for nanostructuring, we
performed an SEM study of laser-induced surface modifi-
cations following ablation with a large variety of F and N.
The obtained data are summarized in FIG. 19. One can see
that the most favorable conditions for pure nanostructuring
are ablation at low and medium values of laser fluence
(F<1.5 J/em?). FIG. 19 also shows the range of laser
irradiation parameters where femtosecond laser ablation
produces different combinations of surface nano-, micro-,
and macro-structures.

To determine the mechanism of nanostructuring, we per-
formed a SEM study on the origin of nanoscale modifica-
tions. A representative example of nascent nanostructures
following ablation with F=0.35 J/em? and N=1 is shown in
FIG. 20(4), where the characteristic types of initial nano-

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structures are labeled. For comparison, FIG. 20(a) shows an
undamaged area of the sample using the same scale as in
FIG. 20(4). It is seen in FIG. 20(4) that surface structuring
is initiated on random, highly-localized nanoscale sites. The
typical structures include circular nanopores with a diameter
in the range of 40-100 nm, randomly-oriented nanoprotru-
sions with a diameter in the range of 20-70 nm and a length
of 20-80 nm, nanocavities of arbitrary form, and nanorims
around nanocavities. Under these femtosecond laser pro-
cessing conditions, nanoscale features down to a size of 20
nm are produced. One can see from FIG. 20(6) that a
nanopore or nanocavity is always immediately accompanied
by a nanorim or nanoprotrusion, indicating a nanoscale
material relocation to an adjacent site. These one-to-one
nanoscale dips and protrusions occur randomly over the
laser spot, suggesting an initial non-uniform laser energy
deposition. Possible factors responsible for the spatial varia-
tion of the absorbed laser energy include: (1) the spatial
inhomogeneity of the incident beam; (2) the enhancement of
absorption by surface defects; (3) interference of the inci-
dent laser light with the excited surface electromagnetic
waves due to structural defects. When the incident laser
fluence is close to the laser ablation threshold, the spatial
variations in deposited laser energy can produce a melt at
localized nanoscale sites within the irradiated spot. Once the
localized nanoscale melts have been formed, a high radial
temperature gradient in a nanomelt can induce a radial
surface tension gradient that expels the liquid to the periph-
ery of the nanomelt. This will lead to the formation of
nanocavities, nanoprotrusions, and nanorims due to fast
freezing of the expelled liquid on the boundary with the solid
state material (see FIG. 20(4)). This mechanism may also be
used to explain the formation of nanobumps on a thin metal
film. These initially induced surface random nanostructures
can enhance the absorption of laser light and facilitate the
further growth of surface nanoroughness due to the
increased spatial non-uniform energy absorption. When
laser fluence is sufficiently high to produce ablation, the
atoms ejected from the nanomelts produce a recoil pressure
that squirts liquid metal outside of the nanomelt. For multi-
pulse ablation, the repeating vaporization and re-deposition
of nanoparticles back onto the surface may also affect the
surface nanostructuring. SEM morphology study at high
fluence (F>5 J/cm; i.e., strong ablation) shows that melt
occurs over a large area of the ablated spot (see FIG. 18) and
the flow dynamics in this large melt pool predominantly
results in microstructuring. We have also studied the ambi-
ent gas pressure effect on nanostructuring by taking SEM
images of platinum following single-pulse ablation in 1-atm
air and in a vacuum at a base pressure of 8x10~* Torr.
Although we have observed a greater amount of re-depos-
ited nanoparticles in air than in vacuum, the morphology of
nanostructures is still quite similar under different air pres-
sures. Our study was performed with samples mounted
vertically. It should be noted that the amount of re-deposited
ablated particles back onto the sample surface may be
different when the sample is positioned vertically versus
horizontally, but further studies are required in this aspect of
nanostructuring using fs laser pulses.

Example 4

Laser-induced periodic surface structures (LIPSS) on
solids have been studied in a number of works in the past.
Typically, LIPSS show regular groove structure with a
period on the incident laser wavelength scale and oriented
perpendicularly to the polarization of the incident light.

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29

LIPSS are commonly seen following long pulse irradiation
on a variety of materials, including semiconductors, metals,
and dielectrics.

In contrast to previous work performed mostly at rela-
tively high fluence, we studied the formation of LIPSS on
platinum and gold in a special fluence regime, namely, at
near damage-threshold fluence. We found a unique type of
LIPSS entirely covered with nanostructures. A distinctive
feature of the nanostructure-covered LIPSS (NC-LIPSS) is
that its period is appreciably less than that of the regular
LIPSS whose period is approximately equal to the laser
wavelength at normal incident laser light. The reduced
period of the nanostructure-covered LIPSS is caused by a
significant increase of the real part of the effective refractive
index of the air-metal interface when nanostructures develop
on a metal surface that affects the propagation of excited
surface plasmon polaritons. Nanostructure-covered LIPSS
has a variety of potential applications, such as modifying
optical properties of materials and chemical catalysts where
high surface-to-volume ratio is a crucial factor.

In this experiment, we used an amplified Ti:sapphire laser
system that generates 65-fs laser pulses with energy about 1
mJ/pulse at a 1 kHz repetition rate and with a central
wavelength A=0.8 um. The horizontally-polarized laser
beam is focused onto a vertically standing metal sample in
air at normal incidence. The number of laser shots, N,
applied to the sample is selected with an electromechanical
shutter. We studied the evolution of NC-LIPSS on metals
following irradiation with N=1, 2, 4, 8, 10, 20, 30, 40, 50,
100, 200, 300, 400, 500 pulses at near damage-threshold
fluence. The studied metals were platinum and gold. The
laser fluence of the incident light was varied by changing the
distance between the focusing lens and sample. To measure
the laser pulse energy incident upon the sample, a fraction of
the incident laser beam is split off by a beamsplitter and
diverted to a pyroelectric joulemeter. The morphology of the
produced periodic structures is examined using a scanning
electron microscope (SEM). The surface profile is measured
with an atomic force microscope (AFM). All sample sur-
faces were mechanically polished using 0.1 4m grade alu-
minum oxide powder.

The evolution of surface structures produced on Pt fol-
lowing ablation at near damage-threshold laser fluence of
F=0.16 J/cm? is shown in FIGS. 21(a)-(b). FIG. 21(@)
demonstrates surface random nanoroughness produced after
10 shot ablation. The inset in FIG. 21(a) shows that this
initial surface modification is characterized by nanocavities
and nanoprotrusions of various forms. At N=20, a
microscale periodic pattern starts to form over the initially

produced random nanoroughness, At this stage, only small 5

patches of periodical structures are observed in various
isolated locations within the irradiated spot, referred to
below as intermediate LIPSS. With increasing N, the inter-
mediate LIPSS grow and coalesce into a clear extended
LIPSS with a period of 0.61 um at N=30 (FIG. 21(b). For N
greater than 70 shots, LIPSS starts to disappear gradually in
the central spot area (FIGS. 22(a) and 22(b)). However, clear
NC-LIPSS continue to form in the peripheral area (FIGS.
22(c) and 22(d). Using AFM, the initial undamaged surface
rms roughness is found to be about 5.6 nm after polishing,
and a typical AFM surface profile measurement on Pt is
shown in FIG. 23(a). Following ablation with N=1, 2, and 10
shots, surface rms roughness is found to be about 16.5, 35.2,
and 79.8 nm, respectively. FIG. 23(b) shows typical surface
roughness after N=10 shots. The surface profile of LIPSS
after 30 laser shots is shown in FIG. 24. To gain the insight
of how initial nanoroughness affects the formation of NC-

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2

10)

30

LIPSS, we performed a SEM study of the formation of
NC-LIPSS with samples of different initial surface condi-
tions. We found that the extended LIPSS is produced with a
smaller number of laser shots when the sample has a greater
surface nanoroughness. To understand the material depen-
dency in forming the nanostructure-covered LIPSS, we also
performed a detailed SEM study of surface structural modi-
fications on Au. Our data show that the general trend is
similar for Au and Pt in forming the initial nanoroughness.
The period of nanostructure-covered LIPSS on Au is
observed to be 0.58 um and is also markedly less than the
laser wavelength (FIG. 25). However, the periodic patterns
on Au are much less clear compared to Pt. Recently, we
performed a comparison study on regular LIPSS on various
metals following femtosecond laser radiation where LIPSS
shows distinctly different level of morphological clearness
among various metals even under identical experimental
conditions. The electron-phonon energy coupling coefli-
cient, g, is shown to directly correlate to the morphological
clearness of LIPSS. A larger g coefficient usually leads to
more pronounced LIPSS. In this study, g coefficient for Pt
and Au are 25x10'® and 2.1x10'° W/m°K, respectively, and
the much larger g coeflicient explains why LIPSS is much
more clear on Pt than Au.

The periodic patterns induced by femtosecond laser pro-
cessing are distinctly different from those produced by
longer pulses in two aspects. First, femtosecond laser-
induced periodic structures are covered by random nano-
structures. Secondly, the LIPSS period induced by femto-
second pulses at normal incidence is appreciably less than
the laser wavelength while the period is roughly equal to the
wavelength for longer pulses. To account for our observa-
tion, we carefully examined the evolution of surface struc-
tural modifications on both Pt and Au, and we propose the
following mechanism for the formation of NC-LIPSS. The
first few laser shots usually produce sparsely and randomly
distributed nanostructures. It is known that surface plas-
mons, both localized and propagating along a surface, can be
excited by coupling laser energy into nanostructures. With
further increase of the number of laser shots, more nano-
structures appear allowing excitation of more localized and
propagating surface plasmons. The produced nanoroughness
includes nanorods, nanocones, and nanospheres, and these
nanostructures will excite propagating cylindrical surface
plasmons that subsequently interfere with the incident light.
This interference causes the formation of intermediate peri-
odie surface microstructures. As the number of laser shots
increases, the intermediate microstructures will grow as well
as the area occupied by these structures. The developed
intermediate periodic surface microstructures will further
excite propagating plane surface plasmons that interfere
with the plane incident laser light wave, and this interference
will finally result in the permanent extended periodic micro-
structures.

For normally incident linearly polarized light, the period
d of the surface grating formed due to the interference
between the incident laser light and the excited surface
plasmon wave is given by equation | as:

a-Wy a)

with gE, where ) is the incident light wavelength, y=Re
[EXE+1)]!” is the real part of the effective refractive index
of the air-metal interface for surface plasmons, € is the
dielectric constant of the metal, g is the grating vector, and
E is the electrical field vector of the incident wave. For a
plane vacuum-metal interface, 1 is calculated to be 1.0096
at A=800 nm for Pt (E,=-15.5 and €,=23.5) and 1.022 for

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31

Au (€,=-23.4, €,=1.55). Using Eq. (1), the grating period
is found to be 0.79 jm for Pt and 0.78 um for Au. However,
the observed period is 0.61 jum for Pt (FIG. 1 (4)) and 0.58
jun for Au (FIG. 5). If we substitute these values of the
observed period into Eq. (1), we will have 7=1.31 for Pt and
= 1.38 for Au. To explain this discrepancy, we note that the
table values of €, and €, for Pt and Au are obtained from
smooth surface and at room temperature, and therefore these
values may not be suitable when the metals are heated by
high-intensity femtosecond pulses and covered with nano-
and micro-structures. To better understand the high-intensity
effects on NC-LIPSS period, we performed a detailed study
of LIPSS in various locations within the damaged spots on
metals. From these data summarized in Table 1, we can see
that the NC-LIPSS period remains the same in the central
and peripheral areas of an irradiated spot despite the fact that
the two locations have different intensities due to the Gauss-
ian beam profile. On the other hand, the period of our
NC-LIPSS decreases with increasing N when the surface
roughness grows while the light intensity remains constant.
Furthermore, the NC-LIPSS produced using a higher fluence
of 0.16 J/cm? exhibit a similar period as that produced at
F=0.084 /em?. Our observations indicate that the high-
intensity effect on dielectric constant is not essential,
whereas the effects of surface morphology (nano- and
micro-roughness) are more dominant. It is known that
surface roughness causes an increase in the modulus of the
surface plasmon wave vector, and this will correspond to an
increase in the real part of the refractive index. According to
Eq. (1), an increased real part of the refractive index for
propagating surface plasmons will cause a reduced NC-
LIPSS period, which agrees with our experimental obser-
vation.

TABLE 1

‘Nanostructure-covered LIPSS period in different areas of the
irradiated spot on platinum at F = 0.084 J/em?.
LIPSS period (um)

Number of shots Central area Peripheral area
30 0.62, 0.61
50 0.58 0.61
100 0.57 057
200 0.55 0,54
500 0.55 0.53

Under certain conditions we also produced a large number
of nanoprotrusions and nanocavities on a metal surface (see
FIG. 22). The nanostructures produced can greatly increase

the effective surface area, which may be of importance in 5

many technological applications, such as but not limited to
producing better chemical catalysts where a high surface-
to-volume ratio is a crucial factor.

Example 5

In this study we performed a femtosecond laser surface
treatment of titanium to help determine the potential of this
technology for surface structuring of titanium implants. We
found find that femtosecond laser processing produces a
large variety of nanostructures (nanopores, nanoprotrusions)
with a size down to 20 nm, multiple parallel grooved surface
patterns with a period on the sub-micron level, microrough-
ness in the range of 1-15 jum with various configurations,
smooth surface with smooth micro-inhomogeneities, and
smooth surface with sphere-like nanostructures down to 10
nm. Also, we have determined the optimal conditions for

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producing these surface structural modifications. Femtosec-
ond laser treatment may produce a richer variety of surface
structures on titanium for implants and other biomedical
applications than long-pulse laser treatments.

Due to good biostability, biocompatibility, mechanical
performance, and long-term durability, titanium has been
widely used in a variety of biomedical applications such as
dental and orthopedic implants, and implantable electronic
devices. In numerous in vitro and in vivo studies, surface
topography of titanium implants has been shown to be
important in enhancing implant performance. It has been
shown that both microstructures and nanostructures influ-
ence biological processes at implant interfaces. Various
methods of implant surface structuring have been studied in
the past such as grit-blasting, chemical etching, laser treat-
ment, and the combinations of the various methods. Recent
studies have shown that laser processing of implant surfaces
provides both suitable surface topography and less surface
contamination as compared with other methods. Another
advantage of laser processing is that the technique is also
suitable for texturing of implants of more complicated
shapes. In the past, surface structures have been produced
using long-pulse lasers, including nanosecond Nd:YAG
laser, copper vapor laser, nanosecond excimer lasers, pico-
second Nd:YAG laser, and sub-picosecond excimer laser.
Femtosecond lasers have advantages over nanosecond lasers
in aspects of higher precision, reduced heat-affected zone,
and smaller amount of debris around the ablated spot.

The effects of laser fluence and the number of applied
pulses on laser-induced surface topography in titanium are
reported herein. We found that a femtosecond laser produces
a large variety of nanostructures (nanopores, nanoprotru-
sions) with a size down to 20 nm, multiple parallel grooved
surface patterns with a period on the sub-micron level,
microroughness in the range of 1-15 jm with various
configurations, smooth surface with smooth micro-inhomo-
geneities, and smooth surface with sphere-like nanostruc-
tures down to 10 nm. Our results suggest that femtosecond
laser treatment can produce a richer variety of surface
structures on titanium for implants and other biomedical
applications than long-pulse laser treatments.

Commercially pure titanium flat plates with a dimension
of 15x17x1.5 mm were used in our experiment. The plates
were mechanically polished using 0.1-um-grade aluminum

5 oxide powder and further cleaned with acetone. For surface

texturing, we used an amplified Ti:sapphire laser system that
generates 65-fs laser pulses with the pulse energy over 1 mJ
at a 1 kHz repetition rate with a central wavelength of 0.8
um. The laser beam is horizontally polarized and was
focused at normal incidence onto a vertically standing
titanium sample in air at a pressure of 1 atm. For laser beam
focusing, we used an achromatic lens with a focal length of
20 cm. The laser fluence of the incident light was varied by
changing the distance between the focusing lens and the
sample. The diameter of laser-irradiated spots on the tita-
nium sample was varied from 100 to 1200 jum. The number
of laser shots, N, applied to the sample was selected with an
electromechanical shutter. The surface structuring of tita-
nium was studied following the treatment with laser fluence
of F=0.067, 0.084, 0.16, 0.35, 0.48, and 2.9 J/cm? and the
number of applied pulses, N, in the range of 1-30,000.
Following femtosecond laser treatment, the topography of
surface modifications was studied using a SEM.

As a reference, FIG. 26(a) shows a SEM image of the
titanium surface prior to laser irradiation. FIGS. 26(5)
through 26(@) demonstrate surface topography produced by
femtosecond laser processing at near-damage-threshold flu-

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33

ence of F=0.067 J/cm? for different numbers of laser shots,
where the characteristic features are random nanopores and
sphere-like nanoprotrusions with the size down to about
15-20 nm. Laser-induced surface nano-topography depends
on both the number of applied pulses and laser fluence. At
higher fluence of F=0.084 J/cm?, the nanoroughness pro-
duced is shown in FIGS. 27(a) through 27(d), where the
average size of the nanostructures at this higher fluence is
larger than those at lower fluence in FIG. 1. For N>10-15,
periodic ordering of surface nanoroughness begins to occur.
FIGS. 28 and 29 show some typical periodic patterns for
laser fluences F=0.067 and 0.084 J/cm?, respectively. The
period of the grooves is about 0.53 um. These periodic
patterns with sub-micron periods are covered with nanor-
oughness, as shown in detail in FIGS. 28(d) and 29(d). With
increasing laser fluence, the periodic patterns are less likely
produced and microroughness becomes a more dominant
surface structure. FIG. 30 shows surface topography pro-

duced following treatment at F=0.16 J/cm? at various N. At 3

this middle-level laser fluence, pure nanoroughness is
observed only after one-shot laser processing (FIG. 30(a)).
Acclear microscale roughness covered with nanoroughness
develops after 20-shot treatment (FIG. 30()). With further
increasing N, microroughness continues to develop with
deepening of cavities (FIG. 30(c)). At a large enough N,
columnar surface micro-structures covered with nanorough-
ness are seen in FIG. 30(d). At higher laser fluence of F=0.35
J/em?, a combination of nano- and micro-structures is pro-
duced after only one laser shot, as shown in FIG. 31(a). With
increasing N, columnar microstructures rapidly develop as
the dominating structures (see FIGS. 31(4)-31(d)). When the
laser fluence is increased to the level of F=0.48 J/cm?, a
different type of surface microstructures is observed, as
shown in FIGS. 32(a)-32(c). At this laser fluence and for
N>1000, a pore of the size of the focused laser beam can be
created. An example of such a pore with the diameter of 350
um is shown in FIG. 32(d), where microstructures are also
seen at bottom of the pore. At the highest fluence used in our
experiment, F=2.9 J/em?, one laser shot can produce surface
melting over the entire irradiated surface area, and resolidi-
fication of this surface melt results in a smooth surface
covered with some micro-inhomogeneities as shown in FIG.
33(a). Following two-pulse irradiation, an even smoother
surface is seen in FIG. 33(4). A magnified picture showing
nanoscale features of such smooth surfaces is shown in FIG.
33(c). A detail picture of the titanium surface after four laser
shots is shown in FIG. 33(d), where one can see nanoscale
structures as small as down to 10 nm. The smooth surface is

produced with a low number of laser shots (N<10). At a 5

larger N(N>10), micro-inhomogeneities develop rapidly and
eventually a crater of the size of the focused laser beam will
be formed.

Tt has been shown that implant surface topography is an
important factor affecting the behaviors of both proteins and
cells on implant surfaces. It is generally accepted that
proteins typically respond to surface structural features (pits,
pillars, steps) about 1-10 nm, while cells can be sensitive to
structural features on the scale of 15 nm-100 um. It was also
found that structured implants have a better mechanical
interlocking of the bone-implant interfaces than smooth
implant surfaces due to an increased surface area. Also, it
has been reported that extended parallel groove structures
may cause cells to align and migrate along the grooves, a
contact guidance phenomenon. Our SEM study shows that
all of these types of surface textures can be produced by
femtosecond laser treatment.

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Little work has been done on laser fabrication of surface
nanostructures on titanium. Our study shows that femtosec-
ond laser technique can produce a large variety of both pure
nanostructures (FIGS. 26(6)-26(d), 27(a)-27(d), and 33(c))
and various combinations of micro- and nanostructures
(FIGS. 28(d), 29(d), 30, 31). There are two types of pure
nanostructures observed in our experiment. The first type
(FIGS. 26(b)-26(d), 27(a)-27(d)) is produced at low laser
fluence (near the damage threshold) and a low number of
laser shots; the size of these nanostructures is down to 20
um. The second type (FIGS. 33(c) and 33(@)) is produced at
high fluence and low N when laser irradiation causes the
surface to melt uniformly over the entire irradiated area; the
size of these nanostructures is down to 10 nm

Examination of shot-to-shot SEM images of surface
topography suggests the following mechanism for the for-
mation of nanostructures of the first type. It is seen from
FIG. 26(d) that a nanopore is always accompanied by a
nearby nanoprotrusion, indicating a nanoscale material relo-
cation to an adjacent site. This one-to-one nanoscale pores/
protrusions relationship occurs randomly over the laser spot,
suggesting an initial non-uniform laser energy deposition.
When the incident laser fluence is close to the laser damage
threshold, spatial non-uniformity in the deposited laser
energy can produce a melt at localized nanoscale sites within
the irradiated spot. Once the localized nanoscale melt has
been formed, a high radial temperature gradient in a nano-
melt can induce a radial surface tension gradient that expels
the liquid to the periphery of the nanomelt. This can lead to
the formation of nanocavities and nanoprotrusions due to
fast freezing of the expelled liquid on the boundary with the
solid state material. These initially induced surface random
nanostructures enhance the absorption of laser light and
facilitate further growth of surface nanoroughness with
increasing number of laser shots due to the increased spatial
non-uniform energy absorption. When laser fluence is suf-
ficiently high to produce ablation, particles will be ejected
from the nanomelts and produce a recoil pressure that squirts
the liquid metal outside of the nanomelt. It should be noted
that for multi-pulse ablation, the repeating vaporization and
re-deposition of nanoparticles back onto the surface can also
promote surface nanostructuring. As seen from FIGS. 26 and
27, the average size and density of femtosecond laser-
induced nanostructural features can be controlled by varying,
both the laser fluence and number of laser shots.

Mechanisms for the formation of nanostructures of the
second type cannot be straightforwardly derived from our
SEM study. The formation of these nanostructural features
may be due to redeposition of ablated nanoparticles back
onto the irradiated surface.

Multiple parallel grooved surface patterns for biomedical
applications are commonly produced using lithographic or
laser holographic techniques. However, fabrication of these
type of patterns on biomaterials using a single laser beam
has not been reported. Below we discuss the optimal con-
ditions for producing these structures and explain the physi-
cal mechanisms of their formation.

Our study shows that optimal conditions for producing
periodic groove patterns on titanium are at near-damage-
threshold fluence and with the laser shot number in the range
between 20 and 800. In the past, multiple parallel grooved
surface patterns have been produced by long-pulse lasers
and are known as laser-induced periodic surface structures
(LIPSS). The formation of LIPSS on metals is believed to
result from the interference of the incident laser light with
the excited surface plasmon polaritons that result in spatial
periodic energy distribution on the surface. Usually, LIPSS

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35

shows a regular groove structure with a period on the
incident laser wavelength scale and is oriented perpendicu-
larly to the polarization of the incident light. Our results of
the evolution of surface structural modifications on titanium,
suggest the following mechanism for the formation of the 5
observed LIPSS. In our experiment, the first few laser shots
produce sparsely and randomly distributed nanostructures. It
is known that propagating cylindrical surface plasmons can
be excited by coupling laser energy into nanoroughness, and
this can give rise to their interference with the incident light.
This interference will, first, cause the formation of interme-
diate periodic surface structures in localized areas of the
irradiated spot. An example of such intermediate periodic
surface structure can be seen in FIG. 26(c). With further
increasing number of laser shots, the number of intermediate
periodic structures will grow as well as the area occupied by
these structures. The developed intermediate periodic sur-
face structures will further excite propagating plane surface
plasmons and their interference with the plane incident laser
light wave will, finally, result in the permanent extended 2
periodic grating.

For linearly polarized incident laser light, the period d of
the surface grating formed due to the interference between
the incident laser light wave and the excited surface plasmon
wave is given by d=A/(n+sin 9) with gl|E, where in this
equation A is the incident light wavelength, n—Re[/
(€+1)]'” is the real part of the effective refractive index of
the air-metal interface for surface plasmons, e is the dielec-
tric constant of the metal, 6 is the laser light incidence angle,
g is the grating vector, and E is the electrical field vector of 30
the incident wave. The above equation shows that the period
of laser-fabricated grating can be varied by changing the
laser wavelength, the incidence angle, or the real part of the
effective refractive index. An important parameter affecting
the cell behavior is known to be groove depth, and this 3
parameter in fabricating LIPSS can be controlled by the
number of applied laser shots.

A unique feature of the periodic groove structures pro-
duced according to the embodiments herein is that both
ridges and grooves are covered with nanoroughness follow-
ing femtosecond laser treatment, in contrast to rectangular
surface grooves fabricated using lithography techniques that
usually have smooth ridges and rough floors.

Laser microtexturing of titanium has been studied in the
past using long-pulse lasers. It has been shown that laser 4:
processing of implant surfaces provides both suitable sur-
face microstructures and the least surface contamination as
compared with other methods. As shown in FIGS. 30(})-
30(d), 31(@)-31(d), 32(a)-32(c), and 33(a), a rich variety of
microstructures can be produced by femtosecond laser treat-  5¢
ment, and these structures can be characterized as the
following two types. The first type (see FIGS. 30(4)-30(d),
31(a)-31(d), 32(a)-32(c)) is produced at the middle levels of
the laser fluence (F=0.16 and 0.35 J/cm). The characteristic
size of this type of microroughness is in the range of 1-15
yum. Both the characteristic size and configuration of the
surface microroughness can be controlled by both laser
fluence and the number of applied shots. This type of
microroughness seems to be only produced by femtosecond
laser treatment. The second type of microroughness (see 6
FIG. 33(a)) is characterized by a smooth surface with
smooth micro-inhomogeneities. This type of microrough-
ness is produced at the highest laser fluence in our experi-
ment (F=2.9 Jem?) when melting occurs over the entire
irradiated area. If the melted surface has some structural
inhomogeneities, fast resolidification of this melted surface
may result in smooth micro-scale roughness.

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Some parts of implant surfaces may be required to be
smooth. Previously, nanosecond excimer lasers have been
used for polishing machined titanium implants, and effects
of both polishing and cleaning of the surfaces have been
reported). Our study shows that smoothed surface can be
also obtained with femtosecond laser treatment, as shown in
FIG. 33(6).

It is known that open pores with a diameter in the range
of 100-400 jum can improve the strength of bone-implant
interfaces. Recently, long-pulse lasers have been used for
fabricating 100-300 tum pores on Ti6AI4V implants. Our
study shows that pores of this size can be produced with
femtosecond laser treatment, as shown in FIG. 32(d). We
note that we can further produce various surface structures
on the pore bottom through femtosecond laser treatment.

Example 6

Another application of femtosecond laser surface struc-
turing to produce the materials processing of the presently
embodied invention is to provide the controllable modifica-
tion of the optical properties of metals, where these optical
properties range from the UV to THz spectral range, and
where the modifications may be used to create various black,
grayed, and colored metals.

As an example, FIG. 34 shows the % reflectance from
0.25 to 2.5 wm of “black” aluminum obtained by the
materials processing methods of the present invention. In the
visible this aluminum appears pitch black as illustrated in
FIG. 35(a)

By varying the materials processing parameters, we have
also produced aluminum that appears to be various shades of
gray. Thus in the case of the grayed aluminum as shown in
FIG, 35(4), the materials processing was performed at laser
fluence F=7.9 /cm*, a scanning speed of the laser beam
across the surface of the Al of v=1 mm/s, and translation
between scanning lines S=100 jum. The two gray shades of
aluminum shown in FIG. 35(6) are obtained by varying the
laser pulse repetition rate (f=100 Hz for the darker shade and
93 Hz for the lighter one). The spectral reflectance of this
darker gray aluminum sample is shown in FIG. 34.

In addition to producing various shades of gray as dis-
cussed above, the materials processing methods of the
present invention can also produce colored metals; i.e.,
metals that appear to have a particular color or that appear
to have multiple colors.

To produce colored metals, two types of femtosecond
laser processing techniques were performed. The first tech-
nique involved tailoring laser-induced surface random struc-
tures, while the second technique produced femtosecond
laser-induced periodic surface structures (FLIPSS). The
colored metals produced by the first technique exhibit the
same apparent color at various viewing angles, while the
colored metals produced by the second technique exhibit
different colors at different viewing angles due to a grating
effect.

FIG. 35(c) shows a picture of a colored aluminum sample
produced by the controlled tailoring of random surface
roughness. The aluminum appears golden in color because
the tailored surface structures preferentially enhance the
absorption at blue and green wavelengths. The spectral
reflectance of the golden aluminum is shown in FIG. 34.

Colored metals produced by the second technique,
FLIPSS, exhibit different colors at different viewing angles.
FIG. 36 shows various colors of an aluminum sample
structured with FLIPSS under experimental conditions of
F=0.05 em?,f=-83 Hz, v=1 mm/s, and $=100 um. The

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spectral reflectance of the color aluminum structured with
FLIPSS is shown in FIG. 34. Structuring with FLIPSS can
cause a polarization effect on the absorption of light that
provides an additional way for controlling the optical prop-
erties. The size of the optically modified metal surface area
can be as small as a tightly focused laser spot; ic. down to
about 10 um, or as large as desired by using a scanning laser
beam (for example, FIGS. 35 and 36 show samples with
structurally modified area of about 24 mm in diameter).

Given the additional advantages of laser processing such
as low contamination and capability to process complicated
shapes, the black, grayed, and colored metals created by
femtosecond laser surface structuring have numerous poten-
tial applications in such areas including, but not limited to,
photonics, plasmonics, optoelectronics, stealth technology,
thermal radiation sources, solar cell absorbers, radiative heat
transfer devices, infrared sensing, bio-optical devices, ther-
mophotovoltaics, and airborne/space borne devices.
Superwicking and Superwetting

Wetting properties of a solid surface are characterized by
the contact angle of a water/liquid drop deposited on the
horizontal surface of the solid as shown in FIG. 37. The
surface can be smooth or structured. A surface is called
hydrophilic when it exhibits the water contact angle smaller
than 90 degrees. The smaller the contact angle, the better the
wetting. A surface is commonly referred to as superhydro-
philic (or superwetting) when the water/liquid spreads to
zero or nearly a zero contact angle. When a smooth surface
is originally hydrophilic, any surface structure produced on
the surface will enhance the hydrophilicity (wettability).

‘Wicking’ means that the surface has capillary properties.
In non-limiting, illustrative aspects of the invention, capil-
lary properties are due to microgrooves engineered into the
surface of the material. The capillary effect in a pipe is well
known. However, when the pipe is cut along its axis into a
half-pipe, the capillary effect will occur as well, albeit to a
much lesser extent than in the full pipe.

Strictly speaking, most any material surface structure has
capillary properties because most any structure can be
viewed as a two-dimensional network of micro- or nano-
channels. However, most surface structures exhibit negli-
gible capillary effect unless they are specifically engineered
to do so. Therefore both smooth and structured surfaces can
be superwetting/superhydrophilic with a water contact angle
close to zero: however, they will not have capillary proper-
ties and are not capable of wicking. To make the surface
wicking, we produce engineered surface structures that are
capable of generating strong capillary forces. Non-limiting,
exemplary engineered surface structures are an array of

parallel microgrooves that can be reduced to a single chan- 5

nel if necessitated by a particular design application. Similar
to non-wicking surfaces, the water/liquid contact angle on
wicking surfaces also can be zero or close to zero, and we
can say that they are superhydrophilic/superwetting.

The femtosecond laser-produced microgrooves disclosed
herein generate strong capillary action that enables water,
for example, to run vertically (against gravity) uphill. This
degree of capillary effect in pipes and grooves is observed
when the material is hydrophilic. The surface of the embod-
ied microgrooves is covered with a hierarchical surface
structure composed of fine microroughness in the form of
protrusions, cavities, spheres, rods, and/or other irregularly
shaped features having heights and/or widths on the order of
0.5 to 100 microns, and nanoroughness in the form of
protrusions, cavities, spheres, rods, grooves, and/or other
irregularly shaped features having heights and/or widths on
the order of 1 to 500 nanometers. As mentioned above, most

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any surface structure on an originally hydrophilic surface
will enhance the original hydrophilicity. The novel hierar-
chical surface structure that covers the surface of the embod-
ied engineered microgrooves significantly enhances the
hydrophilicity of the microgroove surface and, this hierar-
chical surface structure makes the microgroove surface
superhydrophilic/superwetting since the values of the water
contact angle, 6, are close to or substantially zero degrees.
The capillary force is proportional to cos 6: therefore, when
00, the capillary effect achieves a maximum effect under
the same other conditions.

Similarly for the other embodiments disclosed herein, the
laser parameters for controlling the formation of appropriate
surface structures include laser fluence, number of laser
shots N, focused spot diameter, scanning speed, pulse rep-
etition rate, scanning step, laser light polarization, incidence
angle of the laser beam, laser pulse duration, spatial intensity
profile, wavelength, kind and pressure of ambient medium.
To produce any surface structure, laser fluence must exceed
the material’s ablation threshold. Thus as one skilled in the
art will appreciate, the range of laser fluence is any value of
laser fluence above the particular ablation threshold. The
range of number of laser shots (which may be overlapped),
N is Nz. The focused laser spot diameter determines the
width of the microgrooves. Typically, the smallest laser spot
diameter is about 3-5 jm; however, using tightly focused
spots (3-5 um) having a Gaussian spatial intensity profile,
100 nm diameter holes can be produced at ablation threshold
fluence. Scanning the sample across the laser beam can then
produce nanogrooves having widths down to about 100 nm.
Since increasing the laser spot diameter leads to decreasing
the laser fluence, the upper limit on the laser spot diameter
is governed by the value of laser ablation threshold laser
fluence. However, this is not limiting for producing wider
grooves. For example, we can produce a 1 mm-width groove
using 100 m-diameter spots using overlapped scan lines.
Other parameters can be tuned for achieving strong capillary
action depending on material being processed.

Arrays of parallel microgrooves (one-dimensional grat-
ing) in the material’s surface provided unidirectional spread-
ing of liquid. Two-dimensional gratings exhibited uniform
two-dimensional spreading of the liquid. Although experi-
ments were performed with straight grooves, curvilinear or
other shaped groove forms are expected to be equally as

5 effective.

The embodied femtosecond processing methods for cre-
ating superwicking and superwetting materials are or appear
to be suitable for metals, semiconductors, dielectrics
(glasses), polymers, enamels, hard biological tissues (teeth,
bones, etc.) and other materials containing hydroxyapatite.

Wetting properties of solids can be also modified through
surface chemistry. For example, a silicon structured surface
becomes superhydrophobic after coating with a monolayer
of dimethyldichlorosilane [(CH3)2SiC12] reagent. There-
fore, the embodied technique for producing wicking sur-
faces can be supplemented with chemical treatment for
further improving the superwicking and superwetting per-
formance of appropriate materials.

The production of the fine microstructures described
hereinabove may be made or controlled by a variety of
method modifications; that is to say, once a material has
been selected and the general ablation parameters have been
determined, alterations of or in the material’s surface can be
performed in a variety of ways. For example, a desired
indentation in the material in the form of a crater may be
made at time t,. At time t,, another desired indentation may
be made in the surface; and so on and so forth where At may

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be seconds, hours, days, weeks, and so on. Thus, for
example, a two dimensional array of grooves containing fine
microstructural and nanostructural features thereon may be
created in a single step process (as appropriate) or a multi-
step process, with none or various actions between the steps,
as we believe a person skilled in the art will readily
understand.

According to a non-limiting, illustrative two step process,
in a first step, we produce grooves in the surface of the
material of interest using relatively high laser fluence. In a
second step, we reduce the laser fluence to a value most
favorable for producing nano- and micro-structures and
process the surface again. Relatively low values of laser
fluence may be more favorable for producing nanorough-
ness, while relatively moderate values may be more favor-
able for producing a combination of nanoroughness and fine
microroughness. Multiscale surface structures, for example,
may be more efficient for modifying hydrophilicity or
hydrophobicity. Multiscale fine structures may thus be more
favorable when seeking to enhance the wettability of the
grooved surface. We are able to produce non-wicking
(strictly speaking, slightly wicking because any surface
structure can be considered as a 2D network of capillary
channels) structures in the form of microgrooves. To pro-
duce wicking structures on the microgroove surface, we can
set the number of overlapping pulses and laser fluence in the
second step to values that are favorable for producing
laser-induced periodic structures (LIPSS; LIPSS are 1D
nanogratings), as discussed elsewhere herein.

Typically, their structure is an array of parallel nano-
grooves having a width of about 200-300 nm, a depth of
about 100 nm, and a period of about 500-600 nm, for the
fundamental wavelength of a Ti-sapphire laser. Furthermore,
the surface of LIPSS is extensively covered with fine
nanostructures. Since the direction of LIPSS nanogrooves
depends on the light polarization direction, we can produce
LIPSS nanogrooves that are parallel to the microgrooves.
Superwicking

The following is a non-limiting, illustrative description of
superwicking embodiments of the invention in a glass
material. Glass has been widely used in traditional fluidic
devices and more recently in optofluidic devices. The behav-
ior of liquids on a solid surface is determined by the surface
wettability, which can be controllably modified through
engineered surface structuring. According to an embodied
aspect of the femtosecond laser structuring method, we
created novel surface patterns that transform a regular glass
surface into a superwicking material surface for water,
which in a gravity defying way, enabled water to sprint

vertically upwardly along the structured glass surface at an 5

unprecedented velocity of 3.8 cm/sec, following a square
root of time dependence.

Experimental Setup

To create the embodied structural alteration of a glass
surface, we used (as described elsewhere herein above) an
amplified Ti:sapphire laser system that generated 65 fs
pulses with energy around 1.1 mJ/pulse at a maximum
repetition rate of 1 KHz with a central wavelength of 800 nm.
The laser beam was horizontally polarized and was focused
normally onto the glass samples mounted vertically on a
translation stage. The samples were microscope glass slides
with a dimension of 25x25x1 mm? We produced a 20 mm
long microgroove along the horizontal direction by scanning
the sample across the laser beam, followed by a vertical shift
of the sample by 100 uum. This process was repeated to create

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an extended array of parallel microgroove structures. The
resulting structured area was 20x9 mm2. A scanning electron
microscope (SEM) and three dimensional (3D) laser scan-
ning microscope from Keyence were used to examine the
surface structures following the femtosecond laser treat-
ments. Contact angle measurements were performed with a
VCA 2500XE video contact angle system.

Experimental Results and Discussion

A photograph of the laser treated glass sample is shown in
FIG. 38(a). SEM images of the surface structures created on
the glass surface are shown in FIGS. 38(b-d). FIG. 38(b)
shows that the treated surface has multiple parallel micro-
grooves with a period of 100 wm, corresponding to the
vertical step between two horizontal scanning lines. More
detailed surface structural features are shown in FIGS. 38(c)
and (d), where both ridges and valleys of the microgrooves
are covered with nano- and fine micro-structures. As seen
from FIG. 38(d), the nanostructures include both nanopillars
and nanocavities, while the fine microstructures include
microcavities and microscale aggregates from nanoparticles
that fuse onto each other and on the glass surface. FIG. 39
shows a 3D optical image of the treated surface.

We studied the wetting properties of the structured glass
sample both along and perpendicular to the groove orienta-
tion. Distilled water was used in our study, and we recorded
the water spreading dynamics on the structured surface
using a camera. FIGS. 40(a-/) show the wetting dynamics of
a3 ul water droplet pipetted on the horizontally positioned
structured glass surface. For comparison, the behavior of a
3 ul water droplet pipetted on an untreated glass surface is
also shown in FIGS. 40(a-/). As shown, the water drop
spread highly anisotropically on the treated area and it
flowed preferentially along the microgrooves. We repeated
the experiments with different volumes of water in the range
of 1-6 wl, all of which showed a similar highly anisotropic
wetting behavior. When the glass slide was stood vertically
with the grooves oriented parallel to the table, the highly
anisotropic water spreading behavior remained the same,
and we observed no noticeable downward flowing. This can
be clearly seen from FIGS. 41(a-/). From FIG. 40(6) we can
also deduce that the average initial velocity of water spread-
ing is about 5.8 cm/s within the first 0.2 s. As shown in FIGS.

5 40(c-f), the water spreading velocity decreased with time.

We next oriented the glass slide with the grooves perpen-
dicular to the table. When we pipetted a water droplet on the
bottom of the groove area, the water immediately sprinted
vertically uphill (against gravity), as shown in FIGS. 42(a-/).
It can be seen from the figures that this gravity-defying
uphill motion extends over several centimeters. We noted
that the water spread to the very top of the sample and we
expect that the water would continue to spread higher with
a taller sample. This experiment clearly demonstrated that
we transformed a regular glass surface into a superwicking
surface having a capillary driving force much stronger than
gravity. From FIGS. 42(a-/) we deduced that the average
water spreading velocity was about 3.8 cm/sec within the
first 0.2 s, slightly lower than the water spreading velocity
along the horizontal grooves.

Wetting of textured surfaces is commonly explained by
the classical Wenzel and Cassie models (R. N. Wenzel, Ind.
Eng. Chem. 28, 988 (1936); A. B. D. Cassie and S. Baxter,
Trans. Faraday Soc. 40, 546 (1944)). The Wenzel model
assumes that a liquid penetrating into a surface texture will
completely wet the surface, whereas in the Cassie model the
liquid does not fill the texture and there are air pockets

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41

between the liquid and the textured solid surface. The liquid
drop is very adhesive in the Wenzel regime, while it will roll
easily on the surface at small tilts in the Cassie regime. In the
Wenzel model, the wetting is described by relation cos 0*=r
cos 0, where 0* is the apparent contact angle on a rough
surface, r is the roughness factor (the ratio of the actual
surface area to the geometrically projected area on the
horizontal plane), and @ is the contact angle on a smooth
horizontal surface of the same material. Since r is always
greater than 1, the surface texture will enhance the hydro-
philicity of an originally hydrophilic surface (6<90°) and
enhances the hydrophobicity of an originally hydrophobic
surface (0>90°). In our study, the contact angle 6 for water
on a smooth glass surface before the laser treatment was
measured to be 15° and, therefore, the smooth glass surface
is originally hydrophilic (6<90°). According to the Wenzel
model, a surface texture should enhance the hydrophilicity
of the glass. In our experiment, the apparent contact angle
for the laser treated surface was found to be essentially 0°.
Therefore the surface structure produced here turns the glass
surface superhydrophilic. As demonstrated in FIGS. 40-42,
the water drop deposited onto our textured surface is imme-
diately sucked into the texture and water spreads quickly
along the microgrooves for an extended distance from the
point of deposition.

In the past, liquid flow has been studied in capillary
systems such as tubes, open surface grooves, and two
dimensional arrays of pillars. In 1921, Washburn (E. W.
Washburn, Phys. Rev. 17, 273 (1921) showed that motion of
a wetting liquid in a capillary tube follows a diffusion law as
2(aD(t)"?, where z is the distance traveled by the liquid, t
is the time, and D is the diffusion constant. Following
Washburn’s work, behaviors of wetting liquids have been
studied for open capillary systems such as surface grooves
and two-dimensional arrays of pillars. Rye et al. (R. R. Rye,
J. A. Mann, and F. G. Yost, Langmuir 12, 555 (1996)) have
shown that the wicking dynamics in open V-shaped grooves
also follows the Washburn-type t'? dependence as z°=K(a,
8)[yho/t]t, where K(a, 6) is the geometry term with a and
6 being the groove angle and the contact angle, y and 1 are
the surface tension and viscosity of the liquid, and hy is the
groove depth. Therefore, a structured surface can be viewed
as a network of open capillaries, where the liquid spreading
from a reservoir usually follows the Washburn-type scaling
law za(Dt)'?, where D is the diffusion constant. Deviations
from the t’? dynamics have been observed at short initial
stages of the fluid motion in tube capillaries, where fluid
flow exhibits z a t? and z @ t dependences before reaching
the Washburn behavior. To determine the imbibition dynam-

ics of our ultrafast laser-structured surface, we plotted the 5

uphill travel distance z as a function of t!? for the vertically
standing sample, as shown in FIG. 43. One can see that the
spreading distance linearly depends on t’ for our glass
surface despite its complex surface geometry. Similar
spreading dynamics are also observed on the horizontally
positioned sample. We believe that the superwicking action
of the structure created here is due to combined capillary
effects of open microgrooves and finer structures at nano-
and fine micro-scale superimposed on the microgrooves.
Superwetting

The following is a non-limiting, illustrative description of
superwetting embodiments of the invention in dentin and/or
enamel materials.

Good wettability of enamel and dentin surfaces is an
important factor in enhancing adhesion of restorative mate-
rials in dentistry. In general, the wetting of a solid surface by
a liquid depends on three major factors: (1) the surface

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energy of the solid and the liquid; (2) the viscosity of the
liquid; and (3) the surface topography of the solid. There-
fore, surface texturing of the enamel and dentin surfaces is
one of the approaches for improving the wettability and
consequently, bonding strength. Furthermore, the surface
texturing increases the surface area, which enhances
mechanical interlocking between a restorative material and
enamel or dentin. At the present time, etching with an acidic
or basic solution is a widely used approach for surface
texturing in adhesive dentistry, having known disadvan-
tages.

According to the embodied invention, a femtosecond
laser is used to texture the surface of hard dental tissues that
makes both the enamel and dentine surfaces superwetting. In
contrast to the traditional chemical etching which yields a
random surface roughness, the embodied technique pro-
duces an engineered surface structure with a strong capillary
action that enables controllable modification of the wetting
in any extent between the initial wetting and superwetting.
In an illustrative aspect, the engineered surface structure is
an array of parallel microgrooves that generates a strong
capillary force. Due to the powerful capillary action, water
is immediately sucked into this engineered surface structure
and spreads even on a vertical surface against gravity at a
high speed of about 20 mm/s. The embodied approach for
controllable improving the wettability of a dentin/enamel
material can be extended to bones (because both (human)
teeth and bones are mainly composed of hydroxyapatite) and
may be also used for hydroxyapatite coatings of implants,
for example. The embodied method for modifying the
wettability is also suitable for a variety of biocompatible
materials used in dentistry, medicine, biomedicine, and
biosensing.

The superwetting work disclosed herein below was per-
formed on extracted caries-free human molars. Prior to
femtosecond laser treatment, enamel/dentine surfaces were
flattened with 240-, 600-, 1000-, and 2000-grit SiC abrasive
papers. After sanding, the teeth were rinsed in distilled water
and stored in distilled water up to the laser treatment. For
surface texturing of enamel/dentine specimens, we used an
amplified Ti:sapphire laser system that generated 65-fs
pulses with energy around 1.2 mJ/pulse at a maximum
repetition rate of 1 kHz at a central wavelength of 800 nm.
The laser beam was horizontally polarized and was focused
normally onto the specimen mounted vertically on a trans-
lation stage. We scanned the specimen across the laser beam
to produce a 6 mm long microgroove along the horizontal
direction followed by a vertical shift of the specimen by 100
tum. This process was repeated to create an extended array of
parallel microgrooves. A surface area textured with the
microgroove pattern was 2.5x6 and 2.2x6 mm? on the
enamel and dentin specimens, respectively. To obtain
desired wetting properties, the surface structure was tailored
by varying laser fluence, pulse repetition rate, scanning
speed, and focused laser spot diameter.

A scanning electron microscope (SEM) and 3D laser-
scanning microscope VK-9700 from Keyence Co were used
to examine the surface structures following femtosecond
laser treatments. The wetting properties of the dental hard
tissues are reportedly often tested using water contact angle
measurements. We also tested the wettability of the treated
specimens by measuring the contact angle 6 of a distilled
water drop with a volume of 1 pl. The water contact angle
on both the laser treated and untreated surfaces was mea-
sured by the sessile-drop method using a VCA 2500XE

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US 10,876,193 B2

4B

video contact angle system. The spreading dynamics of
distilled water on the treated surface was studied using a
video camera.

Photographs of the laser treated enamel and dentine
specimens are shown in FIGS. 44(a) and 45(a), respectively.
The 3D optical images of the surface structures produced on
the enamel and dentine specimens are shown in FIGS. 44(6)
and 45(5), respectively. The treated surfaces have multiple
parallel microgrooves with a period of 100 yum, correspond-
ing to the step between two laser scanning lines. The depth
of the microgrooves on the enamel and dentine specimens is
about 120 um and 100 um, respectively. The SEM images in
FIGS. 44(c), (d) and 45(c), (d) show fine structural details of
the microgroove surface. The water contact angle before the
laser treatment was measured to be 42° and 48° on the
enamel and dentine specimens, respectively. The contact
angle on the laser-treated surface was found to be ~0° for
both the enamel and dentin specimens. A liquid wets a solid
surface when its contact angle on the surface is smaller than
90°. Commonly, a surface is referred to superwetting (or
superhydrophilic) when the water/liquid spreads to zero or
nearly zero contact angle. Therefore the illustrative surface
structures turn both the enamel and dentin surfaces super-
wetting.

Snapshots in FIGS. 46(a) and 46(5) show the spreading
dynamics of a 1 pl water droplet pipetted on the horizon-
tally-positioned enamel surface, where one can see that the
water rapidly spreads over the laser-treated area. As seen in
FIG. 46(6), the water spreads for a distance of about 4.5 mm
in 0.2 s. From these data, we can deduce that an average
velocity of water spreading is about 22.3 mm/s within the
first 0.2 s. The spreading dynamics of a 1 yl water droplet
pipetted on the vertically-positioned enamel surface with the
microgrooves oriented perpendicular to the table is shown in
FIGS. 46(c) and 46(d), where it is seen that the water
immediately sprints vertically uphill against gravity. From
FIGS. 46(c) and 46(d), we can infer that the average water
spreading velocity is about 21.5 mm/s within the first 0.2
sec, slightly lower than that on the horizontal enamel sur-
face. Similar superwetting behavior of the water is also
observed on the laser-treated dentin specimen as shown in
FIG. 47 for horizontal and vertical orientations of the dentin
surface. FIGS. 47(a) and 47(6) show that the horizontally-
oriented treated surface is completely wetted at t-0.2 s.
From FIGS. 47(c) and 47(d), we can derive that the average
water spreading velocity on the vertical dentin surface is
about 21.7 mm/s within the first 0.2 s. Our data clearly show
that the water completely wets a large structured area within
a fraction of a second.

Human enamel and dentin are heterogeneous substances, 5

dentin more especially than enamel, which consists of 95%
hydroxyapatite (Ca,,(PO,).(OH),), 4% water, and 1% col-
lagen fibers. The dentin is highly heterogeneous and con-
tains 70% hydroxyapatite, 20% collagen fibers, and 10%
water. Moreover, the dentin also has a structural complexity
due to the dentinal tubules. As a consequence, the wettability
modification of the dentin using the commonly known
chemical approaches is a much more complicated procedure
than that for the enamel. In contrast to the common chemical
approaches, the embodied method easily improves the wet-
tability of both the enamel and dentin.

Previously reported behavior of wetting liquids in open
capillary systems such as surface grooves has shown that the
capillary effect in the surface grooves depends on their
geometry. For example, it has been reported that the liquid
spreading in open V-shaped grooves is given by the relation
2-K(a,6)[yho/u]t. where z is the spreading distance, K(a,0)

S

a

w

40

4

B

44

is the geometry term with a and 0 being the groove angle
and the contact angle, y and y are the surface tension and
viscosity of the liquid, hy is the groove depth, and t is the
time. Therefore, varying the geometry of the grooves allows
the controllable modification of the wetting in any extent
between the initial wetting and superwetting. In addition to
a regular 1D-array of capillary microgrooves, other net-
works of capillary channels (regular or irregular) for
improving the wettability can be designed and fabricated
(for example, a regular 2D-array of microgrooves with a
uniform liquid spreading in all directions).

Previous studies on applications of femtosecond lasers in
dentistry have demonstrated such advantages as (i) minimal
collateral damage (thermal and mechanical), (ii) high pre-
cision, (iii) producing microcrack-free cavities, (iv) the
absence of a chemical change in the treated dental hard
tissues, and (v) high processing controllability through a
spectroscopic feedback. The embodied invention demon-
strates that this list can be expanded by the ability of the
femtosecond laser to improve significantly the wettability of
the dental hard tissues for enhanced bonding through pro-
ducing engineered surface structures.

In another demonstrative application, high-intensity fem-
tosecond laser pulses were used to create a superwetting
surface pattern on platinum and gold plates, all having a
dimension of 25x25 mm? The structured metals were cre-
ated with an amplified Ti:sapphire laser that generated 65 fs
pulses with energy around 1.1 mJ/pulse at a maximum
repetition rate of 1 kHz at a central wavelength of 800 nm.
The samples were mounted vertically on a translation stage.
The laser beam was horizontally polarized and was focused
normally onto the samples. We produce an extended area of
surface structures by scanning the samples across the laser
beam along the horizontal direction followed by a vertical
shift. This process was repeated to obtain a structured
circular area of 24 mm in diameter. A scanning electron
microscope (SEM) was used to examine surface structures
following femtosecond laser treatment.

FIG. 48 shows typical surface pattern created on the
metals following femtosecond laser treatment. FIG. 48(a)
shows that the surface has multiple parallel microgrooves
with a period of 100 um, corresponding to the vertical step
between two horizontal scanning lines. As shown in FIGS.
48(b-d), a combination of porous nanostructures and fine
microstructures are superimposed on both ridges and valleys
of the grooved pattern. From FIGS. 48(c) and (@), one can
see that the nanostructures include nanoprotrusions and
nanocavities, while fine microstructures include microcavi-
ties and microscale aggregates of nanoparticles that fuse
onto each other and on the metal surface. As further illus-
trated in FIG. 49, the structured surfaces appear pitch black,
indicating a significant change in optical properties of the
studied metals as reported herein above.

The surface wetting properties of the structured platinum
were studied by positioning the sample horizontally, verti-
cally, and titled at a 45° angle. The liquid used was metha-
nol. The spreading dynamics of methanol on the structured
surfaces was recorded using a video camera at a speed of 30
frames/s. FIGS. 49(a-d) show the wetting dynamics for a
large drop of methanol pipetted on the horizontal surface of
the structured platinum. The liquid can be seen to spread
highly anisotropically and preferentially flow along the
surface microgrooves. Similar experiments with smaller
methanol drops at a volume in the range of 1-10 il also
showed similar highly anisotropic wetting responses. To
determine whether the wetting anisotropy of the structured
platinum was caused by the parallel microgrooves, as shown

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US 10,876,193 B2

45

in FIG. 48(a), we produced another platinum sample with
orthogonally crossed microgrooves. In this case, the metha-
nol spread uniformly along all directions indicating that the
capillary effect in the open microgrooves plays a dominant
role in directionally guiding the liquid.

When we positioned the platinum sample upward at 45°
and vertically at 90°, the drop of methanol moved rapidly
uphill against gravity, as seen in FIGS. 50 (a-d) for the
vertically standing sample. To confirm that the uphill motion
of the liquid was caused by the metal surface structures, we
repeated the above experiments with an untreated platinum
sample and found that the methanol did not flow upward at
all. To study the wetting dynamics, we videotape the liquid
spreading from the moment when methanol was dropped on
the structured metal surface, and FIGS. 49 and 50 show the
frames as the liquid moves with time. We observed that the
average wetting velocity was 1.6, 1.18, and 1 cm/s for the
treated platinum oriented horizontally, 45° upward, and 90°
upward, respectively. We believe that these are the highest
liquid moving speeds upward that one has observed on a
metal surface.

When the bottom part of a vertically standing sample of
structured platinum was submerged in a methanol reservoir,
the methanol spread only along the microgrooves that were
submerged in the methanol, but did not expand laterally to
grooves that were not initially in contact with the methanol
in the reservoir. However, when the sample was enclosed in
a transparent container to suppress evaporation, a similar
vertical wetting strip was formed rapidly, but the liquid also
spread slowly in the lateral directions until the entire struc-
tured black surface became wet. The methanol spreading
speed in the lateral direction was about an order of magni-
tude lower than that along the vertical grooves. Thus in
addition to the engineered capillary effect, evaporation also
plays a role for the unidirectional spreading of methanol in
open air. Therefore, in addition to a physical wetting bound-
ary formed by the outmost groove walls that are in contact
with the methanol reservoir, the evaporation actually creates
a virtual wall leading to a significantly enhanced anisotropic
(essentially unidirectional) wetting behavior. When evapo-
ration was suppressed in the enclosed container, the
observed wetting for the vertically standing black platinum
in all directions showed that the nano- and micro-structures
superimposed on the groove pattern also enhanced the
wicking effect of the platinum specimen.

Another observed property of the structured platinum was
that a significant amount of liquid could be transported
uphill due to a strong capillary pumping effect. To illustrate
this effect, we structured a long stripe of surface area of 2

mm wide and 25 mm long on a platinum foil and bent the 5

foil into an L shape, as shown in FIG. 50(e). We then
immersed the bottom edge of the black stripe in a methanol
reservoir. Within 10 min, a large drop of methanol accumu-
lated at the upper end of the black stripe that was 10 mm
above the methanol reservoir surface, as shown in FIG.
50(/). The accumulated liquid volume was measured to be
about 10 ul, indicating that the structured platinum can draw
a significant amount of liquid against gravity to an elevated
point.

The self-propelled motion of liquids against gravity has
been observed in the past when the surface has a hydropho-
bicity gradient that causes a liquid drop to move from a more
hydrophobic surface area to a less hydrophobic area. In our
experiments, however, there is no hydrophobicity gradient
along the direction of the liquid spreading and therefore, this
mechanism could be ruled out. Another known mechanism
that drives liquids uphill on an inclined surface is the

S

a

w

46

classical “tears of wine” phenomenon, originally observed
in a glass of strong wine and explained by Thomson (J.
Thomson, Philos. Mag. 10(4), 330 (1855). This phenom-
enon is caused by a preferential evaporation of alcohol from
alcohol-water mixture that produces a concentration gradi-
ent and a surface tension gradient that generates a force
driving a liquid film upward on the wine glass wall. The
drawn-up liquid accumulates on the glass walls and then
forms running-down droplets called tears of wine. To deter-
mine whether the vertically upflowing liquid is a similar
evaporation-driven phenomenon in our experiment, we
placed the methanol reservoir with the submerged sample
shown in FIG. 3(/) in a closed transparent container, and
observed that liquid did not accumulate at the top end of the
black metal track any more, although the blackened track
was wetted due to the capillary effect. The accumulation of
methanol on the top end of the blackened track occurred
again as soon as the container was reopened. We thus believe
that the methanol accumulation was due to the evaporation-
driven Marangoni effect, where fluid flow is induced by
surface tension gradients in volatile liquids. Although we
used a single-component volatile liquid, the absorption of
water from the atmosphere can make our liquid become a
two-component mixture to some degree. The fact that the
vertically standing structured metal surface remained con-
stantly wet in a closed container showed that the surface
structures alone have an extraordinarily strong wicking
effect even in the absence of the driving force from the
evaporation.

Table I shows a variety of laser processing parameters for
various materials including platinum (Pt), glass, dentine, and
enamel. Therein below, Table I also lists exemplary engi-
neered features in the various materials in the left column
and dimensional ranges for these features in the right
column. Table I thus provides a recipe that will enable the
skilled person to create the embodied superwicking and/or
superwetting effects in various materials and for various
applications.

TABLE I

Laser processing parameters

Parameter range

Metal sample (Pt)

Laser fluence: 0.05-200 Hem?

Spot diameter: O.1 pm-S em

Pulse duration: 5 fs-continuous wave

Pulse repetition rate: 1 Hz-80 MHz

Scanning speed: 0.1 pm/sec-S emisee

Step between scanning lines: 0.1 m-5 em

Ambient gas: air; inert or chemically
active gases; inert or
chemically active liquids;
Vacuum

Glass sample

Laser fluence: 05-500 Sem?

Spot diameter: O.1 pm-2 em

Pulse duration: 5 fs-contimous wave

Pulse repetition rate: 1 Hz-80 MHz

Scanning speed 0.1 pm/see-2 emisee

Step between scanning lines: 0.1 m-2 em

Ambient gas: air; inert or chemically active

gases; inert or chemically
active liquids; Vacuum
Dentine sample

0.05-50 Tem?
O.1 pm-1 em

Laser fluence:
Spot diameter:

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US 10,876,193 B2

47
TABLE I-continued

Laser processing parameters

Pulse duration:
Pulse repetition rate:
Scanning speed:

Step between scanning lines:
Ambient gas:

Enamel sample

Laser fluence:
Spot diameter:

Pulse duration:

Pulse repetition rate:
Scanning speed:

Step between scanning lines:
Ambient gas:

5 f-continmous wave
1 Hz-80 MHz

0.1 um/sec-1 em/sec
0.1-500 um

air; blowing inert or
chemically active gases

0.05-50 J/em?

0.1 pm-L em

5 fs-continuous wave
1 Hz-80 MHz

0.1 pmisec-1 em/see
0.1-500 um

air, blowing inert or

chemically active gases

Structures Structure range

Grooves on metal (Pt)

Period: 100 um 10 nm-10 em
Width: 100 pm 10 om-5 mm
Depth: 75 um 10 om-5 mm
Grooves on glass
Period: 100 jum 10 nm-10 em
Width: 100 pm 10 nm-$ mm
Depth: 40 ym 10 nm-5 mm
Grooves on dentin
10 nm-2 mm
5 10 nm-2 mm
Depth: 100 jun 10 om-2 mm
Grooves on enamel
Period: 100 um 10 om-2 mm
idth: 100 jm 10 nm-2 mm
Depth: 120 ym 10 nm-2 mm
Fine microroughness
covering the groove surface of
metal, glass, dentin, and enamel
Size: 0.5-10 um 0.5-100 im

Shape: various protrusion, cavity, sphere, rod,
other izregular shepes
Nanoroughness covering

the groove surface of metal,

glass, dentin, and enamel

Size: 5-500 nm.
Shape: various

1-500 nm
protrusion, cavity, sphere, rod,
and other irregular shapes

All references, including publications, patent applica-
tions, and patents, cited herein are hereby incorporated by

reference to the same extent as if each reference were ~

individually and specifically indicated to be incorporated by
reference and were set forth in its entirety herein.

The use of the terms “a” and “an” and “the” and similar
referents in the context of describing the invention (espe-
cially in the context of the following claims) are to be
construed to cover both the singular and the plural, unless
otherwise indicated herein or clearly contradicted by con-
text. The terms “comprising,” “having,” “including.” and
“containing” are to be construed as open-ended terms (i.
meaning “including, but not limited to,”) unless otherwise
noted. The term “connected” is to be construed as partly or
wholly contained within, attached to, or joined together,
even if there is something intervening.

The recitation of ranges of values herein are merely
intended to serve as a shorthand method of referring indi-
vidually to each separate value falling within the range,

S

w
8

w

30

35

60

48

unless otherwise indicated herein, and each separate value is
incorporated into the specification as if it were individually
recited herein.

All methods described herein can be performed in any
suitable order unless otherwise indicated herein or otherwise
clearly contradicted by context. The use of any and all
examples, or exemplary language (e.g., “such as”) provided
herein, is intended merely to better illuminate embodiments
of the invention and does not impose a limitation on the
scope of the invention unless otherwise claimed.

No language in the specification should be construed as
indicating any non-claimed element as essential to the
practice of the invention.

It will be apparent to those skilled in the art that various
modifications and variations can be made to the present
invention without departing from the spirit and scope of the
invention. There is no intention to limit the invention to the
specific form or forms disclosed, but on the contrary, the
intention is to cover all modifications, alternative construc-
tions, and equivalents falling within the spirit and scope of
the invention, as defined in the appended claims. Thus, it is
intended that the present invention cover the modifications
and variations of this invention provided they come within
the scope of the appended claims and their equivalents

We claim:

1. A method for engineering a surface of a material to be
superhydrophilic and to increase a degree of capillary effect
on the surface of the material, the method comprising:

identifying a surface region of the material to increase the

degree of capillary effect;

scanning a first laser spot relative to the identified surface

region to produce a first series of adjacent wicking
microgrooves;

scanning a second laser spot relative to the identified

surface region to produce additional wicking structures
ona surface of the first series of wicking microgrooves,
the additional wicking structures comprising an array
of parallel nanogrooves that are parallel to the first
series of parallel microgrooves;

wherein the first series of adjacent wicking microgrooves

in combination with the array of parallel nanogrooves
that are parallel to the first series of adjacent wicking
microgrooves increases the degree of capillary effect on
the identified surface region.

2. The method of claim 1 wherein the material comprises
a metal.

3. The method of claim 1, wherein material comprises a
non-superhydrophilic glass material prior to scanning the
first and second laser spots relative to the identified surface
region.

4. The method of claim 1, wherein the material comprises
a non-superhydrophilic dielectric material prior to scanning
the first and second laser spots relative to the identified
surface region.

5. The method of claim 1, wherein the material comprises
a non-superhydrophilic semiconductor material prior to
scanning the first and second laser spots relative to the
identified surface region.

6. The method of claim 1, wherein the material comprises
a non-superhydrophilic polymer material prior to scanning
the first and second laser spots relative to the identified
surface region.

7. The method of claim 1, wherein the material comprises
a non-superhydrophilic dentin material prior to scanning the
first and second laser spots relative to the identified surface
region.

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US 10,876,193 B2

49

8. The method of claim 1, wherein the material comprises
an non-superhydrophilic enamel material prior to scanning
the first and second laser spots relative to the identified
surface region.

9. The method of claim 1, wherein the material comprises
non-superhydrophilic hydroxyapatite prior to scanning the
first and second laser spots relative to the identified surface
region.

10. The method of claim 1, wherein the material is
non-superhydrophillic glass prior to scanning the first and
second laser spots relative to the identified surface region
and wherein the microgrooves have a periodicity of 100#5
yum, a width of 1005 jm, and a depth of 40+5 pm.

11. The method of claim 1, wherein the material is
non-superhydrophilic dentin prior to scanning the first and
second laser spots relative to the identified surface region
and wherein the microgrooves have a periodicity of 95+5
jum, a width of 95+5 jum, and a depth of 100+5 ym.

12. The method of claim 1, wherein the material is
non-superhydrophilic enamel prior to scanning the first and
second laser spots relative to the identified surface region
and wherein the microgrooves have a periodicity of 100#5
um, a width of 10025 jum, and a depth of 120+5 jum.

13. The method of claim 1, wherein the scanning steps are
selected from at least one of direct laser ablation, interfero-

a

w

50

metric laser ablation, near-field laser ablation, a mask pro-
jection ablation technique, laser-assisted chemical etching,
deposition from a laser ablation plume, plasmonic nanoab-
lation, and a self-assembled microlens array formed by
deposition of glass microspheres on the material surface.

14. The method of claim 1, wherein scanning the first
laser spot comprises scanning the first laser spot at a first
fluence, wherein scanning the second laser spot comprises
scanning the second laser spot at a second fluence, wherein
the first and second fluences are different.

15. The method of claim 14, wherein the first fluence is
higher than the second fluence.

16. The method of claim 15, wherein the first series of
adjacent wicking microgrooves are produced prior to the
array of parallel nanogrooves being produced.

17. The method of claim 1, wherein at least some of the
additional wicking structures are created by overlapping
laser shots.

18. The method of claim 1, wherein the microgrooves are
created in a two-dimensional array for liquid spreading in
two directions.

19. The method of claim 1, wherein the microgrooves are
created in straight lines.

ee oR wk
Source notes & attribution
  1. https://rexresearch.com/GuoSuperhydrophobicMetal/US10876193B2.pdf

Dossier visual record.

All 1 figures

Source illustrations for Water-repelling metal. Captions identify the document and evidence type.

Keep following.

Thematic connections, not evidence of a shared mechanism